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题名: On the evaluation of diffusivities in gels using the diffusion cell technique
作者: Zhang, W;  Furusaki, S
关键词: diffusion cell ;  alginate gel ;  diffusion ;  immobilized cells ;  the quasi-steady-state method ;  the time-lag method
刊名: BIOCHEMICAL ENGINEERING JOURNAL
发表日期: 2001-11-01
卷: 9, 期:1, 页:73-82
收录类别: SCI
文章类型: Article
部门归属: 18101
项目归属: 1812
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Modeling of the gel-immobilized cell system requires accurate measurement of diffusion coefficients. Three methods of the quasi-steady-state (QSS) method, the time-lag (TL) method and a variant quasi-steady-state (VQSS) method were critically assessed and compared for the evaluation of diffusivities using the diffusion cell technique. Experimental data from our laboratory were used for the analysis of the influence of crucial theoretical assumptions not being fulfilled in each method. The results highlighted a risk in obtaining highly variable diffusion coefficients by not validating the QSS and the accuracy of the measurements. In the TL method, the estimation of diffusivities based on the plot intercept that was mostly used in the literature, results in a many fold lower value when compared to that based on the plot slope. The comparison with the QSS and VQSS methods confirmed similar diffusivity obtained by the TL method based on the plot slope. It thus suggested that the correct estimation of diffusivities by the TL method could be based on the plot slope only. Furthermore, the errors associated with the solute mass in the gel, the sample withdrawal and the non-negligible concentration changes in the chambers were also discussed. It is concluded that diffusion cell technique has to be employed cautiously for a correct evaluation of diffusivities. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: CALCIUM ALGINATE MEMBRANES ;  LACTIC-ACID FERMENTATION ;  IMMOBILIZED CELLS ;  MASS-TRANSFER ;  MALIC-ACID ;  COEFFICIENTS ;  GLUCOSE ;  MODEL ;  LAYERS ;  BEADS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000172057700010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84669
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Flinders Univ S Australia, Sch Med, Bedford Pk, SA 5042, Australia
2.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dept Biochem Engn, Dalian 116023, Peoples R China
3.Kyushu Univ, Dept Chem Syst & Engn, Fukuoka 8128581, Japan

Recommended Citation:
Zhang, W,Furusaki, S. On the evaluation of diffusivities in gels using the diffusion cell technique[J]. BIOCHEMICAL ENGINEERING JOURNAL,2001,9(1):73-82.
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