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题名: Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers
作者: Zhang, Q;  Zou, HF;  Wang, HL;  Ni, JY
关键词: enantiomeric separation ;  chiral stationary phase ;  albumin stationary phase ;  triazine stationary phase
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2000-01-14
卷: 866, 期:2, 页:173-181
收录类别: SCI
文章类型: Article
部门归属: 4
项目归属: 403
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel method of synthesizing protein chiral stationary phase (protein-CSP) is proposed with 2,4,6-trichloro-1,3,5-triazine as the activator. The bovine serum albumin (BSA) based chiral columns (150x4.6 mm I.D.) were prepared successfully within 8 h. With tryptophan as the probe solute, it was observed that the BSA immobilized by this method had a better ability to distinguish enantiomers than that activated by glutaric dialdehyde. This may be due to the well-maintained BSA conformation and the larger amount of BSA immobilized on the silica gel. The BSA-CSP prepared by this method was relatively stable under experimental conditions, and the resolution of 13 chiral compounds was achieved. The coupling reaction in this method is mild, reliable and reproducible; it is also suitable for the immobilization of various biopolymers in the preparation of bioreactor, biosensor and affinity chromatography columns. (C) 2000 Elsevier Science B.V. All rights reserved.
关键词[WOS]: AFFINITY-CHROMATOGRAPHY ;  AMINO-ACIDS ;  SEPARATION ;  HPLC ;  BINDING ;  RECOGNITION ;  TRYPTOPHAN ;  PROTEINS ;  COLUMNS ;  AGAROSE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000084778200002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84965
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Q,Zou, HF,Wang, HL,et al. Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers[J]. JOURNAL OF CHROMATOGRAPHY A,2000,866(2):173-181.
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