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Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers
Zhang, Q; Zou, HF; Wang, HL; Ni, JY
关键词Enantiomeric Separation Chiral Stationary Phase Albumin Stationary Phase Triazine Stationary Phase
刊名JOURNAL OF CHROMATOGRAPHY A
2000-01-14
866期:2页:173-181
收录类别SCI
文章类型Article
部门归属4
项目归属403
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]AFFINITY-CHROMATOGRAPHY ; AMINO-ACIDS ; SEPARATION ; HPLC ; BINDING ; RECOGNITION ; TRYPTOPHAN ; PROTEINS ; COLUMNS ; AGAROSE
英文摘要A novel method of synthesizing protein chiral stationary phase (protein-CSP) is proposed with 2,4,6-trichloro-1,3,5-triazine as the activator. The bovine serum albumin (BSA) based chiral columns (150x4.6 mm I.D.) were prepared successfully within 8 h. With tryptophan as the probe solute, it was observed that the BSA immobilized by this method had a better ability to distinguish enantiomers than that activated by glutaric dialdehyde. This may be due to the well-maintained BSA conformation and the larger amount of BSA immobilized on the silica gel. The BSA-CSP prepared by this method was relatively stable under experimental conditions, and the resolution of 13 chiral compounds was achieved. The coupling reaction in this method is mild, reliable and reproducible; it is also suitable for the immobilization of various biopolymers in the preparation of bioreactor, biosensor and affinity chromatography columns. (C) 2000 Elsevier Science B.V. All rights reserved.
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WOS记录号WOS:000084778200002
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被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84965
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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Zhang, Q,Zou, HF,Wang, HL,et al. Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers[J]. JOURNAL OF CHROMATOGRAPHY A,2000,866(2):173-181.
APA Zhang, Q,Zou, HF,Wang, HL,&Ni, JY.(2000).Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers.JOURNAL OF CHROMATOGRAPHY A,866(2),173-181.
MLA Zhang, Q,et al."Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers".JOURNAL OF CHROMATOGRAPHY A 866.2(2000):173-181.
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