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Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers
Zhang, Q; Zou, HF; Wang, HL; Ni, JY
KeywordEnantiomeric Separation Chiral Stationary Phase Albumin Stationary Phase Triazine Stationary Phase
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2000-01-14
Volume866Issue:2Pages:173-181
Indexed BySCI
SubtypeArticle
Department4
Funding Project403
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordAFFINITY-CHROMATOGRAPHY ; AMINO-ACIDS ; SEPARATION ; HPLC ; BINDING ; RECOGNITION ; TRYPTOPHAN ; PROTEINS ; COLUMNS ; AGAROSE
AbstractA novel method of synthesizing protein chiral stationary phase (protein-CSP) is proposed with 2,4,6-trichloro-1,3,5-triazine as the activator. The bovine serum albumin (BSA) based chiral columns (150x4.6 mm I.D.) were prepared successfully within 8 h. With tryptophan as the probe solute, it was observed that the BSA immobilized by this method had a better ability to distinguish enantiomers than that activated by glutaric dialdehyde. This may be due to the well-maintained BSA conformation and the larger amount of BSA immobilized on the silica gel. The BSA-CSP prepared by this method was relatively stable under experimental conditions, and the resolution of 13 chiral compounds was achieved. The coupling reaction in this method is mild, reliable and reproducible; it is also suitable for the immobilization of various biopolymers in the preparation of bioreactor, biosensor and affinity chromatography columns. (C) 2000 Elsevier Science B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000084778200002
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84965
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Q,Zou, HF,Wang, HL,et al. Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers[J]. JOURNAL OF CHROMATOGRAPHY A,2000,866(2):173-181.
APA Zhang, Q,Zou, HF,Wang, HL,&Ni, JY.(2000).Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers.JOURNAL OF CHROMATOGRAPHY A,866(2),173-181.
MLA Zhang, Q,et al."Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers".JOURNAL OF CHROMATOGRAPHY A 866.2(2000):173-181.
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