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题名: Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase
作者: Wang, HL;  Zou, HF;  Ni, JY;  Kong, L;  Gao, S;  Guo, BC
关键词: Artemisia capillaris ;  scoparone ;  capillarisin
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2000-02-18
卷: 870, 期:1-2, 页:501-510
收录类别: ISTP ;  SCI
文章类型: Article
部门归属: 4
项目归属: 403
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A method for the screening and analysis of biologically active compounds in traditional Chinese medicine is proposed. Affinity chromatography using a human serum albumin (HSA) stationary phase was applied to separate and analyze the bioactive compounds from Artemisia capillaris Thunb. Five major peaks and several minor peaks were resolved based on their affinity to HSA, two of them were identified as scoparone (SCO, 6,7-dimethoxycoumarin) and capillarisin (CAP). CAP shows a much higher affinity to HSA than SCO. The effects of acetonitrile concentration, eluent pH, phosphate concentration and temperature on the retention behaviors of several major active components were also investigated, and it was found that hydrophobicity and eluent pH play major roles in changing retention values. The results demonstrate that the affinity chromatography with a HSA stationary phase is an effective way for analyzing and screening biologically active compounds in traditional Chinese medicine. (C) 2000 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ALLOSTERIC INTERACTION ;  PROTEIN-BINDING ;  PLASMA-PROTEIN ;  SAIBOKU-TO ;  MICRODIALYSIS ;  DRUGS ;  ENANTIOMERS ;  WARFARIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000085455900054
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84985
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China
2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA

Recommended Citation:
Wang, HL,Zou, HF,Ni, JY,et al. Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase[J]. JOURNAL OF CHROMATOGRAPHY A,2000,870(1-2):501-510.
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