DICP OpenIR
Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase
Wang, HL; Zou, HF; Ni, JY; Kong, L; Gao, S; Guo, BC
KeywordArtemisia Capillaris Scoparone Capillarisin
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2000-02-18
Volume870Issue:1-2Pages:501-510
Indexed ByISTP ; SCI
SubtypeArticle
Department4
Funding Project403
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; ALLOSTERIC INTERACTION ; PROTEIN-BINDING ; PLASMA-PROTEIN ; SAIBOKU-TO ; MICRODIALYSIS ; DRUGS ; ENANTIOMERS ; WARFARIN
AbstractA method for the screening and analysis of biologically active compounds in traditional Chinese medicine is proposed. Affinity chromatography using a human serum albumin (HSA) stationary phase was applied to separate and analyze the bioactive compounds from Artemisia capillaris Thunb. Five major peaks and several minor peaks were resolved based on their affinity to HSA, two of them were identified as scoparone (SCO, 6,7-dimethoxycoumarin) and capillarisin (CAP). CAP shows a much higher affinity to HSA than SCO. The effects of acetonitrile concentration, eluent pH, phosphate concentration and temperature on the retention behaviors of several major active components were also investigated, and it was found that hydrophobicity and eluent pH play major roles in changing retention values. The results demonstrate that the affinity chromatography with a HSA stationary phase is an effective way for analyzing and screening biologically active compounds in traditional Chinese medicine. (C) 2000 Elsevier Science B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000085455900054
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84985
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China
2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA
Recommended Citation
GB/T 7714
Wang, HL,Zou, HF,Ni, JY,et al. Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase[J]. JOURNAL OF CHROMATOGRAPHY A,2000,870(1-2):501-510.
APA Wang, HL,Zou, HF,Ni, JY,Kong, L,Gao, S,&Guo, BC.(2000).Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase.JOURNAL OF CHROMATOGRAPHY A,870(1-2),501-510.
MLA Wang, HL,et al."Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase".JOURNAL OF CHROMATOGRAPHY A 870.1-2(2000):501-510.
Files in This Item:
File Name/Size DocType Version Access License
2000R1F26GX23T.pdf(248KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, HL]'s Articles
[Zou, HF]'s Articles
[Ni, JY]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, HL]'s Articles
[Zou, HF]'s Articles
[Ni, JY]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, HL]'s Articles
[Zou, HF]'s Articles
[Ni, JY]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.