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Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase
Wang, HL; Zou, HF; Ni, JY; Kong, L; Gao, S; Guo, BC
关键词Artemisia Capillaris Scoparone Capillarisin
刊名JOURNAL OF CHROMATOGRAPHY A
2000-02-18
870期:1-2页:501-510
收录类别ISTP ; SCI
文章类型Article
部门归属4
项目归属403
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ALLOSTERIC INTERACTION ; PROTEIN-BINDING ; PLASMA-PROTEIN ; SAIBOKU-TO ; MICRODIALYSIS ; DRUGS ; ENANTIOMERS ; WARFARIN
英文摘要A method for the screening and analysis of biologically active compounds in traditional Chinese medicine is proposed. Affinity chromatography using a human serum albumin (HSA) stationary phase was applied to separate and analyze the bioactive compounds from Artemisia capillaris Thunb. Five major peaks and several minor peaks were resolved based on their affinity to HSA, two of them were identified as scoparone (SCO, 6,7-dimethoxycoumarin) and capillarisin (CAP). CAP shows a much higher affinity to HSA than SCO. The effects of acetonitrile concentration, eluent pH, phosphate concentration and temperature on the retention behaviors of several major active components were also investigated, and it was found that hydrophobicity and eluent pH play major roles in changing retention values. The results demonstrate that the affinity chromatography with a HSA stationary phase is an effective way for analyzing and screening biologically active compounds in traditional Chinese medicine. (C) 2000 Elsevier Science B.V. All rights reserved.
语种英语
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WOS记录号WOS:000085455900054
引用统计
被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84985
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China
2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA
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Wang, HL,Zou, HF,Ni, JY,et al. Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase[J]. JOURNAL OF CHROMATOGRAPHY A,2000,870(1-2):501-510.
APA Wang, HL,Zou, HF,Ni, JY,Kong, L,Gao, S,&Guo, BC.(2000).Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase.JOURNAL OF CHROMATOGRAPHY A,870(1-2),501-510.
MLA Wang, HL,et al."Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase".JOURNAL OF CHROMATOGRAPHY A 870.1-2(2000):501-510.
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