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Separation of acidic compounds by strong anion-exchange capillary electrochromatography
Ye, ML; Zou, HF; Liu, Z; Ni, JY
关键词Electrochromatography Ion-exchange Electrochromatography Mobile Phase Composition Benzoic Acids
刊名JOURNAL OF CHROMATOGRAPHY A
2000-07-28
887期:1-2页:223-231
收录类别SCI
文章类型Article
部门归属4
项目归属403
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ZONE ELECTROPHORESIS ; GRADIENT ELUTION ; REVERSED-PHASE ; FLOW ; EFFICIENCY ; PARAMETERS
英文摘要Separation of the acidic compounds in the ion-exchange capillary electrochromatograph (IE-CEC) with strong anion-exchange packing as the stationary phase was studied. It was observed that the electroosmotic flow (EOF) in strong anion-exchange CEC moderately changed with increase of the eluent ionic strength and decrease of the eluent pH, but the acetonitrile concentration in the eluent had almost no effect on the EOF. The EOF in Strong anion-exchange CEC with eluent of low pH value was much larger than that in RP-CEC with Spherisorb-ODS as the stationary phase. The retention of acidic compounds on the strong anion-exchange packing was relatively weak due to only partial ionization of them, and both chromatographic and electrophoretic processes contributed to separation. It was observed that the retention values of acidic compounds decreased with the increase of phosphate buffer and acetonitrile concentration in the eluent as well as the decrease of the applied voltage, and even the acidic compounds could elute before the void time. These factors also made an important contribution to the separation selectivity for tested acidic compounds, which could be separated rapidly with high column efficiency of more than 220 000 plates/m under the optimized separation conditions. (C) 2000 Elsevier Science BN. All rights reserved.
语种英语
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WOS记录号WOS:000088315800015
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被引频次:65[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84991
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Lab Chromatog, Dalian 116011, Peoples R China
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Ye, ML,Zou, HF,Liu, Z,et al. Separation of acidic compounds by strong anion-exchange capillary electrochromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2000,887(1-2):223-231.
APA Ye, ML,Zou, HF,Liu, Z,&Ni, JY.(2000).Separation of acidic compounds by strong anion-exchange capillary electrochromatography.JOURNAL OF CHROMATOGRAPHY A,887(1-2),223-231.
MLA Ye, ML,et al."Separation of acidic compounds by strong anion-exchange capillary electrochromatography".JOURNAL OF CHROMATOGRAPHY A 887.1-2(2000):223-231.
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