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题名: Separation of acidic compounds by strong anion-exchange capillary electrochromatography
作者: Ye, ML;  Zou, HF;  Liu, Z;  Ni, JY
关键词: electrochromatography ;  ion-exchange electrochromatography ;  mobile phase composition ;  benzoic acids
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2000-07-28
卷: 887, 期:1-2, 页:223-231
收录类别: SCI
文章类型: Article
部门归属: 4
项目归属: 403
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Separation of the acidic compounds in the ion-exchange capillary electrochromatograph (IE-CEC) with strong anion-exchange packing as the stationary phase was studied. It was observed that the electroosmotic flow (EOF) in strong anion-exchange CEC moderately changed with increase of the eluent ionic strength and decrease of the eluent pH, but the acetonitrile concentration in the eluent had almost no effect on the EOF. The EOF in Strong anion-exchange CEC with eluent of low pH value was much larger than that in RP-CEC with Spherisorb-ODS as the stationary phase. The retention of acidic compounds on the strong anion-exchange packing was relatively weak due to only partial ionization of them, and both chromatographic and electrophoretic processes contributed to separation. It was observed that the retention values of acidic compounds decreased with the increase of phosphate buffer and acetonitrile concentration in the eluent as well as the decrease of the applied voltage, and even the acidic compounds could elute before the void time. These factors also made an important contribution to the separation selectivity for tested acidic compounds, which could be separated rapidly with high column efficiency of more than 220 000 plates/m under the optimized separation conditions. (C) 2000 Elsevier Science BN. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ZONE ELECTROPHORESIS ;  GRADIENT ELUTION ;  REVERSED-PHASE ;  FLOW ;  EFFICIENCY ;  PARAMETERS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000088315800015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84991
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Lab Chromatog, Dalian 116011, Peoples R China

Recommended Citation:
Ye, ML,Zou, HF,Liu, Z,et al. Separation of acidic compounds by strong anion-exchange capillary electrochromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2000,887(1-2):223-231.
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