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题名: Separation of peptides by strong cation-exchange capillary electrochromatography
作者: Ye, ML;  Zou, HF;  Liu, Z;  Ni, JY
关键词: capillary electrochromatography ;  strong cation exchange ;  separation mechanism ;  peptides
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2000-02-11
卷: 869, 期:1-2, 页:385-394
收录类别: ISTP ;  SCI
文章类型: Article
部门归属: 4
项目归属: 403
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Separation of small peptides on ion-exchange capillary electrochromatography (IE-CEC) with strong cation-exchange packing (SCX) as stationary phase was investigated. It was observed that the number of theoretical plates for small peptides varied from 240 000 to 460 000/m, and the relative standard deviation for t(0) and the migration time of peptides were less than 0.57% and 0.27%, respectively for ten consecutive runs. Unusually high column efficiency has been explained by the capillary electrophoretic stacking and chromatofocusing phenomena during the injection and separation of positively charged peptides. The sample buffer concentration had a marked effect on the column efficiency and peak area of the retained peptides. The influences of the buffer concentration and pH value as well as the applied voltage on the separation were investigated. It has been shown that the electrostatic interaction between the positively charged peptides and the SCX stationary phase played a very important role in IE-CEC, which provided the different separation selectivity from those in the capillary electrophoresis and reversed-phase liquid chromatography. A fast separation of ten peptides in less than 3.5 min on IE-CEC by adoption of the highly applied voltage was demonstrated. (C) 2000 Elsevier Science B.V. All rights reserved.
关键词[WOS]: ELECTROSPRAY MASS-SPECTROMETRY ;  PACKED CAPILLARIES ;  PHARMACEUTICAL ANALYSIS ;  LIQUID-CHROMATOGRAPHY ;  STATIONARY-PHASE ;  REVERSED-PHASE ;  ELECTROPHORESIS ;  SILICA ;  EFFICIENCY ;  RESOLUTION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000085401600034
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84993
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Chromatog, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Ye, ML,Zou, HF,Liu, Z,et al. Separation of peptides by strong cation-exchange capillary electrochromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2000,869(1-2):385-394.
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