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题名: Alkaline treatment of the cellulose fiber affecting membrane column behaviour for high-performance immunoaffinity chromatography
作者: Zhou, DM;  Zou, HF;  Wang, HL;  Ni, JY;  Zhang, Q;  Zhang, YK
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2000-12-01
卷: 14, 期:8, 页:511-515
收录类别: SCI
文章类型: Article
部门归属: 4
项目归属: 403
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Pharmacology & Pharmacy
英文摘要: The original cellulose fibers and those treated by alkaline solution were both used to prepare the acrylic membranes. The two kinds of membranes were packed into the columns for high-performance immunoaffinity chromatography by the immobilization of protein A on them. It was observed that the alkaline treatment of the cellulose fiber decreased the pressure resistance of the membrane to the mobile phases and greatly increased the accessible volume to the proteins, but affected the adsorption capacity of human IgG on the protein A membrane columns less. There is little difference between those two kinds of membranes on the adsorption capacities of HIgG, which means that the alkaline treatment of the cellulose fiber only significantly changes the void volume inter-membrane, and the porosity and surface area of membrane less. Alkaline treatment of the cellulose fiber reduced the membrane-column efficiency significantly. Some typical examples for the immunoaffinity analysis of IgG from human and dog plasma on the protein A membrane columns are illustrated. Copyright (C) 2000 John Wiley & Sons, Ltd.
关键词[WOS]: AFFINITY-CHROMATOGRAPHY ;  PURIFICATION ;  SERUM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000166001200002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/85003
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhou, DM,Zou, HF,Wang, HL,et al. Alkaline treatment of the cellulose fiber affecting membrane column behaviour for high-performance immunoaffinity chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,2000,14(8):511-515.
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