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题名: Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives
作者: You, JM;  Zhang, B;  Zhang, WB;  Yang, P;  Zhang, YK
关键词: derivatisation ;  LC ;  alcohols ;  carbazole-9-N-acetic acid
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2001-02-16
卷: 909, 期:2, 页:171-182
收录类别: SCI
文章类型: Article
部门归属: 18084
项目归属: 1810
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Alcohols were derivatised to their carbazole-9-N-acetic acid (CRA) esters with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC . HCl) as the dehydrating agent. Studies on derivatisation conditions indicated that the coupling reaction proceeded rapidly and smoothly in the presence of a base catalyst in acetonitrile to give the corresponding sensitively fluorescent derivatives. The retention behaviour of alcohol derivatives was investigated by varying mobile phase compositions (ACN-water and MeOH-water). The parameters from the equation log k'=A-BX were evaluated by retention data of derivatives using an isocratic elution with different mobile phases. The results indicated that the parameters derived allowed computation of retention factors in good agreement with experiments. At the same time, a general equation was derived that makes possible predictions of partition coefficient in binary mobile phases with different proportions of organic solvent to water based on some simple regression analysis. The LC separation for the derivatised alcohols containing higher carbon alcohols showed good reproducibility on a reversed-phase C-18 column with gradient elution. The detection limits (excitation at 335 nm, emission at 360 nm) for derivatised alcohols (signal-to-noise ratio=3:1) were in the range of 0.1-0.4 pg per injection. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PRECOLUMN FLUORESCENCE DERIVATIZATION ;  ALIPHATIC-ALCOHOLS ;  GAS-CHROMATOGRAPHY ;  HYDROXYL COMPOUNDS ;  REAGENT ;  METHANOL ;  PLASMA ;  SEPARATION ;  RETENTION ;  ETHANOL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000166774400005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/85425
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China

Recommended Citation:
You, JM,Zhang, B,Zhang, WB,et al. Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives[J]. JOURNAL OF CHROMATOGRAPHY A,2001,909(2):171-182.
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