DICP OpenIR
Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives
You, JM; Zhang, B; Zhang, WB; Yang, P; Zhang, YK
KeywordDerivatisation Lc Alcohols Carbazole-9-n-acetic Acid
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2001-02-16
Volume909Issue:2Pages:171-182
Indexed BySCI
SubtypeArticle
Department18084
Funding Project1810
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPRECOLUMN FLUORESCENCE DERIVATIZATION ; ALIPHATIC-ALCOHOLS ; GAS-CHROMATOGRAPHY ; HYDROXYL COMPOUNDS ; REAGENT ; METHANOL ; PLASMA ; SEPARATION ; RETENTION ; ETHANOL
AbstractAlcohols were derivatised to their carbazole-9-N-acetic acid (CRA) esters with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC . HCl) as the dehydrating agent. Studies on derivatisation conditions indicated that the coupling reaction proceeded rapidly and smoothly in the presence of a base catalyst in acetonitrile to give the corresponding sensitively fluorescent derivatives. The retention behaviour of alcohol derivatives was investigated by varying mobile phase compositions (ACN-water and MeOH-water). The parameters from the equation log k'=A-BX were evaluated by retention data of derivatives using an isocratic elution with different mobile phases. The results indicated that the parameters derived allowed computation of retention factors in good agreement with experiments. At the same time, a general equation was derived that makes possible predictions of partition coefficient in binary mobile phases with different proportions of organic solvent to water based on some simple regression analysis. The LC separation for the derivatised alcohols containing higher carbon alcohols showed good reproducibility on a reversed-phase C-18 column with gradient elution. The detection limits (excitation at 335 nm, emission at 360 nm) for derivatised alcohols (signal-to-noise ratio=3:1) were in the range of 0.1-0.4 pg per injection. (C) 2001 Elsevier Science B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000166774400005
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/85425
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China
Recommended Citation
GB/T 7714
You, JM,Zhang, B,Zhang, WB,et al. Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives[J]. JOURNAL OF CHROMATOGRAPHY A,2001,909(2):171-182.
APA You, JM,Zhang, B,Zhang, WB,Yang, P,&Zhang, YK.(2001).Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives.JOURNAL OF CHROMATOGRAPHY A,909(2),171-182.
MLA You, JM,et al."Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives".JOURNAL OF CHROMATOGRAPHY A 909.2(2001):171-182.
Files in This Item:
File Name/Size DocType Version Access License
200191XN026335.pdf(183KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[You, JM]'s Articles
[Zhang, B]'s Articles
[Zhang, WB]'s Articles
Baidu academic
Similar articles in Baidu academic
[You, JM]'s Articles
[Zhang, B]'s Articles
[Zhang, WB]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[You, JM]'s Articles
[Zhang, B]'s Articles
[Zhang, WB]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.