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题名: Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift
作者: Zhang, W;  Jin, MF;  Yu, XJ;  Yuan, Q
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2001-03-01
卷: 55, 期:2, 页:164-169
收录类别: SCI
文章类型: Article
部门归属: 18099
项目归属: 1812
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Repeated-batch cultures of strawberry cells (Fragaria ananassa cv. Shikinari) subjected to four medium-shift procedures (constant LS medium, constant B5 medium, alternation between LS and B5 starting from LS and alternation between LS and B5 starting from B5) were investigated for the enhanced anthocyanin productivity. To determine the optimum period for repeated batch cultures, two medium-shift periods of 9 and 14 days were studied, which represent the end of the exponential growth phase and the stationary phase. By comparison with the corresponding batch cultures, higher anthocyanin productivity was achieved for all the repeated-batch cultures at a 9-day medium-shift period. The average anthocyanin productivity was enhanced 1.7-and 1.76-fold by repeated-batch cultures in constant LS and constant B5 medium at a 9-day shift period for 45 days, respectively. No further improvement was observed when the medium was alternated between LS (the growth medium) and B5 (the production medium). Anthocyanin production was unstable at a 14-day shift period regardless of the medium-shift procedures. The results show that it is feasible to improve anthocyanin production by a repeated-batch culture of strawberry cells.
关键词[WOS]: SUSPENSION-CULTURES ;  DIOSCOREA-DELTOIDEA ;  CATHARANTHUS-ROSEUS ;  GROWTH-KINETICS ;  TEMPERATURE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000167646600005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/85433
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biochem Engn, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Flinders Univ S Australia, Sch Med, Bedford Pk, SA 5042, Australia

Recommended Citation:
Zhang, W,Jin, MF,Yu, XJ,et al. Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2001,55(2):164-169.
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