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Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift
Zhang, W; Jin, MF; Yu, XJ; Yuan, Q
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
2001-03-01
55期:2页:164-169
收录类别SCI
文章类型Article
部门归属18099
项目归属1812
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]SUSPENSION-CULTURES ; DIOSCOREA-DELTOIDEA ; CATHARANTHUS-ROSEUS ; GROWTH-KINETICS ; TEMPERATURE
英文摘要Repeated-batch cultures of strawberry cells (Fragaria ananassa cv. Shikinari) subjected to four medium-shift procedures (constant LS medium, constant B5 medium, alternation between LS and B5 starting from LS and alternation between LS and B5 starting from B5) were investigated for the enhanced anthocyanin productivity. To determine the optimum period for repeated batch cultures, two medium-shift periods of 9 and 14 days were studied, which represent the end of the exponential growth phase and the stationary phase. By comparison with the corresponding batch cultures, higher anthocyanin productivity was achieved for all the repeated-batch cultures at a 9-day medium-shift period. The average anthocyanin productivity was enhanced 1.7-and 1.76-fold by repeated-batch cultures in constant LS and constant B5 medium at a 9-day shift period for 45 days, respectively. No further improvement was observed when the medium was alternated between LS (the growth medium) and B5 (the production medium). Anthocyanin production was unstable at a 14-day shift period regardless of the medium-shift procedures. The results show that it is feasible to improve anthocyanin production by a repeated-batch culture of strawberry cells.
语种英语
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WOS记录号WOS:000167646600005
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/85433
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biochem Engn, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Flinders Univ S Australia, Sch Med, Bedford Pk, SA 5042, Australia
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Zhang, W,Jin, MF,Yu, XJ,et al. Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2001,55(2):164-169.
APA Zhang, W,Jin, MF,Yu, XJ,&Yuan, Q.(2001).Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,55(2),164-169.
MLA Zhang, W,et al."Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 55.2(2001):164-169.
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