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Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy
Jia, JY; Yang, L; Zou, HF; Zhang, YK; Zhao, JY; Fan, CM; Sha, LY
Source PublicationBIOMEDICAL CHROMATOGRAPHY
1999-11-01
Volume13Issue:7Pages:472-477
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS KeywordIMMUNOGLOBULIN-G ; IMMUNE-COMPLEXES ; IGG ; REMOVAL ; ANTIBODIES ; ADSORPTION ; PLASMA
AbstractTangential flow affinity membrane cartridge (TFAMC) fs a new model of immunoadsorption therapy for hemoperfusion. Recombinant Protein A was immobilized on the membrane cartridge through Schiff base formation for extracorporeal IgG and immune complex removal from blood. Flow characteristics, immunoadsorption capacity and biocompatibility of protein A TFAMC were studied. The results showed that the pressure drop increased with the increasing flow rate of water, plasma and blood, demonstrating reliable strength of membrane at high now rare. The adsorption capacities of protein A TFAMC for IgG from human plasma and blood were measured. The cartridge with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23.8 mg IgG/g dry matrix) from human plasma and 499.4 mg IgG (21.5 mg IgG/g dry matrix) from human blood, respectively. The circulation time had a major influence on IgG adsorption capacity, but the flow rate had little influence. Experiments in vitro and in vivo confirmed that protein A TFAMC mainly adsorbed Ige and Little of other plasma proteins, and that blood cell damage was negligible. The extracorporeal circulation system is safe and reliable. Copyright (C) 1999 John Wiley & Sons, Ltd.
Language英语
URL查看原文
WOS IDWOS:000083726000008
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/85569
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dept Renal Internal Med, Dalian 116023, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Rheumatol & Immunol, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Jia, JY,Yang, L,Zou, HF,et al. Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(7):472-477.
APA Jia, JY.,Yang, L.,Zou, HF.,Zhang, YK.,Zhao, JY.,...&Sha, LY.(1999).Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy.BIOMEDICAL CHROMATOGRAPHY,13(7),472-477.
MLA Jia, JY,et al."Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy".BIOMEDICAL CHROMATOGRAPHY 13.7(1999):472-477.
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