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题名: Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy
作者: Jia, JY;  Yang, L;  Zou, HF;  Zhang, YK;  Zhao, JY;  Fan, CM;  Sha, LY
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 1999-11-01
卷: 13, 期:7, 页:472-477
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Pharmacology & Pharmacy
英文摘要: Tangential flow affinity membrane cartridge (TFAMC) fs a new model of immunoadsorption therapy for hemoperfusion. Recombinant Protein A was immobilized on the membrane cartridge through Schiff base formation for extracorporeal IgG and immune complex removal from blood. Flow characteristics, immunoadsorption capacity and biocompatibility of protein A TFAMC were studied. The results showed that the pressure drop increased with the increasing flow rate of water, plasma and blood, demonstrating reliable strength of membrane at high now rare. The adsorption capacities of protein A TFAMC for IgG from human plasma and blood were measured. The cartridge with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23.8 mg IgG/g dry matrix) from human plasma and 499.4 mg IgG (21.5 mg IgG/g dry matrix) from human blood, respectively. The circulation time had a major influence on IgG adsorption capacity, but the flow rate had little influence. Experiments in vitro and in vivo confirmed that protein A TFAMC mainly adsorbed Ige and Little of other plasma proteins, and that blood cell damage was negligible. The extracorporeal circulation system is safe and reliable. Copyright (C) 1999 John Wiley & Sons, Ltd.
关键词[WOS]: IMMUNOGLOBULIN-G ;  IMMUNE-COMPLEXES ;  IGG ;  REMOVAL ;  ANTIBODIES ;  ADSORPTION ;  PLASMA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000083726000008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/85569
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dept Renal Internal Med, Dalian 116023, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Rheumatol & Immunol, Dalian 116023, Peoples R China

Recommended Citation:
Jia, JY,Yang, L,Zou, HF,et al. Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(7):472-477.
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