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Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy
Jia, JY; Yang, L; Zou, HF; Zhang, YK; Zhao, JY; Fan, CM; Sha, LY
刊名BIOMEDICAL CHROMATOGRAPHY
1999-11-01
13期:7页:472-477
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]IMMUNOGLOBULIN-G ; IMMUNE-COMPLEXES ; IGG ; REMOVAL ; ANTIBODIES ; ADSORPTION ; PLASMA
英文摘要Tangential flow affinity membrane cartridge (TFAMC) fs a new model of immunoadsorption therapy for hemoperfusion. Recombinant Protein A was immobilized on the membrane cartridge through Schiff base formation for extracorporeal IgG and immune complex removal from blood. Flow characteristics, immunoadsorption capacity and biocompatibility of protein A TFAMC were studied. The results showed that the pressure drop increased with the increasing flow rate of water, plasma and blood, demonstrating reliable strength of membrane at high now rare. The adsorption capacities of protein A TFAMC for IgG from human plasma and blood were measured. The cartridge with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23.8 mg IgG/g dry matrix) from human plasma and 499.4 mg IgG (21.5 mg IgG/g dry matrix) from human blood, respectively. The circulation time had a major influence on IgG adsorption capacity, but the flow rate had little influence. Experiments in vitro and in vivo confirmed that protein A TFAMC mainly adsorbed Ige and Little of other plasma proteins, and that blood cell damage was negligible. The extracorporeal circulation system is safe and reliable. Copyright (C) 1999 John Wiley & Sons, Ltd.
语种英语
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WOS记录号WOS:000083726000008
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/85569
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dept Renal Internal Med, Dalian 116023, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Rheumatol & Immunol, Dalian 116023, Peoples R China
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GB/T 7714
Jia, JY,Yang, L,Zou, HF,et al. Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(7):472-477.
APA Jia, JY.,Yang, L.,Zou, HF.,Zhang, YK.,Zhao, JY.,...&Sha, LY.(1999).Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy.BIOMEDICAL CHROMATOGRAPHY,13(7),472-477.
MLA Jia, JY,et al."Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy".BIOMEDICAL CHROMATOGRAPHY 13.7(1999):472-477.
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