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题名: Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography
作者: Wang, HL;  Kong, L;  Zou, HF;  Ni, JY;  Zhang, YK
关键词: column liquid chromatography ;  human serum albumin ;  Angelica sinensis (Oliv.) Diels ;  bioactive compounds
刊名: CHROMATOGRAPHIA
发表日期: 1999-10-01
卷: 50, 期:7-8, 页:439-445
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel strategy for the screening and analysis of biologically active compounds in traditional Chinese medicine by molecular biochromatography is proposed. Molecular biochromatography with human serum albumin (HSA) immobilized on silica as stationary phase was used to screen and analyse the bioactive compounds in the typical Chinese medicine of Angelica sinensis (Oliv.) Diels. Ten peaks showed retention on this column, which is based on their affinity for HSA. Ferulic acid and liguistilide were identified as the principal active components, which agrees very well with the results in the literature. A quality control method was also developed based on the simultaneous determination the concentrations of ferulic acid and liguistilide in solutions of Angelica sinensis (Oliv.) Diels extracted with water and methanol. It was observed that the concentrations of ferulic acid and liguistilide in solution extracted with methanol were 2 and 53 times higher, respectively, than those with water. It was shown that molecular biochromatography is an effective way of analysing and screening biologically active compounds in traditional Chinese medicine.
关键词[WOS]: HUMAN-SERUM-ALBUMIN ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ALLOSTERIC INTERACTION ;  STATIONARY PHASE ;  PROTEIN-BINDING ;  PLASMA-PROTEIN ;  SAIBOKU-TO ;  MICRODIALYSIS ;  ENANTIOMERS ;  WARFARIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000084861000008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/85585
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R & A Ctr, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Wang, HL,Kong, L,Zou, HF,et al. Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography[J]. CHROMATOGRAPHIA,1999,50(7-8):439-445.
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