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题名: Drug-protein interaction studied by technique of microdialysis combined with high performance liquid chromatography
作者: Zou, HF;  Wang, HL;  Zhang, YK
刊名: REVIEWS IN ANALYTICAL CHEMISTRY
发表日期: 1999
卷: 18, 期:6, 页:383-407
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Drug-protein binding is an important process in determining the activity and fate of a pharmaceutical agent once it has entered the body. This review examines the method of microdialysis combined with high-performance liquid chromatography (HPLC) that has been developed;by ours to study such interactions, in which the microdialysis was applied to sample the free drug in the mixed solution of drug with protein, and HPLC to quantify the concentration of free drug in the microdialysate. This technique has successfully been used for determining various types of binding interactions between the low affinity drugs, high affinity drugs and enantiomers to HSA. For the case of competitive binding of two drugs to a protein in solution, a displacement equation has been derived and examined with four nonsteroidal anti-inflammatory drugs and HSA as model drugs and protein, respectively. Microdialysis with HPLC was adopted to determine simultaneously the free solute and displacing agent in drug-protein solutions. The method is able to locate the binding site and determine affinity constants even up to 10(7) L/mol accurately.
关键词[WOS]: HUMAN SERUM-ALBUMIN ;  NONSTEROIDAL ANTIINFLAMMATORY DRUGS ;  AFFINITY CAPILLARY-ELECTROPHORESIS ;  PLASMA-PROTEIN ;  BINDING-SITES ;  STEREOSELECTIVE BINDING ;  STATIONARY PHASE ;  FRONTAL ANALYSIS ;  SEPARATION ;  COLUMNS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000085684300002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/85603
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zou, HF,Wang, HL,Zhang, YK. Drug-protein interaction studied by technique of microdialysis combined with high performance liquid chromatography[J]. REVIEWS IN ANALYTICAL CHEMISTRY,1999,18(6):383-407.
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