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题名: New method for analysis of Chinese herbal complex prescription and its application
作者: Xiao, HB;  Liang, XM;  Lu, PZ;  Chan, CK
关键词: Chinese herbal complex prescription ;  black box analysis ;  HPLC unified method ;  Dang-Gui-Bu-Xue-Tang
刊名: CHINESE SCIENCE BULLETIN
发表日期: 1999-07-01
卷: 44, 期:13, 页:1164-1172
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Traditional Chinese medicine (TCM) is a great treasure of China, the analysis of which is an arduous task. The viewpoint that all chemical constituents of Chinese herbal complex prescription should be analyzed as a black box is elucidated for the first time. Intelligent multi-mode multi-column chromatographic system (IMMCC) with its hybrids is the basic method and HPLC Unified Method is the breakthrough for the black box analysis. Dang-Gui-Bu-Xue-Tang was selected as a typical TCM and a systematic separation method from non-aqueous mobile phase to pure water mobile phase was put forward in order to convert unknown sample to known sample. The a, c values and UV spectra of 66 components of Astragalus, 78 components of Angelica and 71 components of Dang-Gui-Bu-Xue-Tang were obtained. Intelligent optimization and peak identification method and software for complex samples were developed and the optimum multi-step multi-binary gradient curve of mobile phase for Astragalus was ascertained. The maximum error and minimum error of predicted retention time for all components of Astragalus are 8.62% and 0.05% respectively. All components of Astragalus were compared with those of Angelica and it is found that many components of Astragalus are the same as those of Angelica, while the contents of these components are different. Many components of Dang-Gui-Bu-Xue-Tang are also the same as those of Astragalus and Angelica with different contents.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000082955000003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/86109
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
2.Govt Lab, Hong Kong, Hong Kong

Recommended Citation:
Xiao, HB,Liang, XM,Lu, PZ,et al. New method for analysis of Chinese herbal complex prescription and its application[J]. CHINESE SCIENCE BULLETIN,1999,44(13):1164-1172.
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