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题名: Determination of total iron binding capacity of serum by capillary electrophoresis
作者: Ding, YS;  Liu, LL;  Ma, YF;  Lin, BC
关键词: capillary electrophoresis ;  serum analysis ;  total iron binding capacity
刊名: CHROMATOGRAPHIA
发表日期: 1999
卷: 49, 期:1-2, 页:71-74
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A capillary electrophoresis (CE) technique for determining total iron binding capacity (TIBC) of serum has been developed. The optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with Fe+3; then, dissociate them completely after removing excess unbound Fe. Finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the CE analysis. Ammonium acetate (pH = 5.0) was used as CE background electrolyte solution. In this system, a good linear correlation coefficient was maintained over the range 0.5 similar to 10 mu M (r = 0.9979, n =12). Seven adult serum samples were studied and the TIBC parameters measured. In the present system, 10 similar to 30 mu L serum is sufficient for determination. The study shows that the CE technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.
关键词[WOS]: TRANSFERRIN RECEPTOR LEVELS ;  RHEUMATOID-ARTHRITIS ;  PERFORMANCE ;  SEPARATION ;  PROTEINS ;  ANEMIA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000078308200012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/86171
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Truman State Univ, Div Sci, Kirksville, MO 63501 USA

Recommended Citation:
Ding, YS,Liu, LL,Ma, YF,et al. Determination of total iron binding capacity of serum by capillary electrophoresis[J]. CHROMATOGRAPHIA,1999,49(1-2):71-74.
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