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Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis
Zhang, B; Fung, YS; Lau, KM; Lin, BC
刊名BIOMEDICAL CHROMATOGRAPHY
1999-06-01
13期:4页:267-271
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]FLUORESCENCE
英文摘要Capillary zone electrophoresis was used to monitor the interaction between bilirubin and human serum albumin. Cord blood serum samples were injected directly into an uncoated fused-silica capillary (30 cm x 50 mu m i.d.) and separation was accomplished within 4 min without extensive sample pretreatment. The most suitable running buffer to separate free bilirubin from albumin bound bilirubin was found to contain 1.0 mmol/L EDTA, 5% acetonitrile and 15 mmol/L phosphate with pH adjusted to 8.4. Approximately two bilirubin dianions could be bound per human serum albumin molecule in the cord blood serum. The binding constant was estimated to be 1.1 x 10(5) (L/mol) at 25 degrees C and pH 8.4. The peak area ratio of free bilirubin to total bilirubin can be used to determine the bilirubin binding capacity of cord blood serum for the concentration range of total bilirubin from 204 to 340 mu mol/L using 1:5 diluted cord blood seras. Copyright (C) 1999 John Wiley & Sons, Ltd.
语种英语
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WOS记录号WOS:000081341200003
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/86219
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Peoples R China
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GB/T 7714
Zhang, B,Fung, YS,Lau, KM,et al. Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(4):267-271.
APA Zhang, B,Fung, YS,Lau, KM,&Lin, BC.(1999).Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis.BIOMEDICAL CHROMATOGRAPHY,13(4),267-271.
MLA Zhang, B,et al."Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis".BIOMEDICAL CHROMATOGRAPHY 13.4(1999):267-271.
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