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题名: A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures
作者: Zhang, W;  Furusaki, S;  Franco, C
关键词: strawberry cell ;  suspension cultures ;  anthocyanin ;  temperature ;  two-stage culture ;  temperature-shift
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 1999-08-01
卷: 42, 期:4, 页:345-350
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A two-stage process with temperature-shift has been developed to enhance the anthocyanin yield in suspension cultures of strawberry cells. The effect of the temperature-shift interval and the shift-time point was investigated for the optimization of this strategy. In this process, strawberry cells were grown at 30 degrees C (the optimum temperature for cell growth) for a certain period as the first stage, with the temperature shifted to a lower temperature for the second stage. In response to the temperature shift-down, anthocyanin synthesis was stimulated and a higher content could be achieved than that at both boundary temperatures but cell growth was suppressed. When the lower boundary temperature was decreased, cell growth was lowered and a delayed, sustained maximum anthocyanin content was achieved. Anthocyanin synthesis was strongly influenced by the shift-time point but cell growth was not. Consequently, the maximum anthocyanin content of 2.7 mg.g-fresh cell(-1) was obtained on day 9 by a temperature-shift from 30 degrees C, after 3-d culture, to 15 degrees C. The highest anthocyanin yield of 318 mg.L-1 on day 12 was achieved when the temperature was shifted from 30 degrees C, after 5-d culture, to 20 degrees C. For a global optimization of both the yield and productivity, the optimum anthocyanin yield and productivity of 272 mg.L-1 and 30.2 mg.L-1.d(-1) on day 9 were achieved by a two-stage culture with a temperature-shift from 30 degrees C after 3 d to 20 degrees C.
关键词[WOS]: CATHARANTHUS-ROSEUS ;  GROWTH
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000082227600002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/87339
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Flinders Univ S Australia, Sch Med, Bedford Pk, SA 5042, Australia
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biochem Engn, Dalian 116012, Peoples R China
3.Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 113, Japan

Recommended Citation:
Zhang, W,Furusaki, S,Franco, C. A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1999,42(4):345-350.
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