DICP OpenIR
A homogeneous DNA hybridization system by using a new luminescence terbium chelate
Sueda, S; Yuan, JL; Matsumoto, K
Source PublicationBIOCONJUGATE CHEMISTRY
2002-03-01
DOI10.1021/bc010049+
Volume13Issue:2Pages:200-205
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordFLUORESCENCE ENERGY-TRANSFER ; DIKETONATE-EUROPIUM CHELATE ; LABEL ; PROBES
AbstractHomogeneous DNA hybridization assay based on the luminescence resonance energy transfer (LRET) from a new luminescence terbium chelate, N,N,N-1,N-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (BPTA)-Tb3+ (lambda(ex) = 325 nm and lambda(em) = 545 nm) to an organic dye, Cy3 (A,. = 548 nm and A,. = 565 nm), has been developed. In the system, two DNA probes whose sequences are complementary to the two different consecutive sequences of a target DNA are used; one of the probes is labeled with the Tb3+ chelate at the T-end, and the other is with Cy3 at the 5'-end. Labeling of the Tb3+ chelate is accomplished via the linkage of a biotin-labeled DNA probe with the Tb3+ chelate-labeled streptavidin. Strong sensitized emission of Cy3 was observed upon excitation of the Tb3+ chelate at 325 run, when the two probe DNAs were hybridized with the target DNA. The sensitivity of the assay was very high compared with those of the previous homogeneous-format assays using the conventional organic dyes; the detection limit of the present assay is about 30 pM of the target DNA strand.
Language英语
URL查看原文
WOS IDWOS:000174592600007
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/91437
Collection中国科学院大连化学物理研究所
Affiliation1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Sueda, S,Yuan, JL,Matsumoto, K. A homogeneous DNA hybridization system by using a new luminescence terbium chelate[J]. BIOCONJUGATE CHEMISTRY,2002,13(2):200-205.
APA Sueda, S,Yuan, JL,&Matsumoto, K.(2002).A homogeneous DNA hybridization system by using a new luminescence terbium chelate.BIOCONJUGATE CHEMISTRY,13(2),200-205.
MLA Sueda, S,et al."A homogeneous DNA hybridization system by using a new luminescence terbium chelate".BIOCONJUGATE CHEMISTRY 13.2(2002):200-205.
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