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A homogeneous DNA hybridization system by using a new luminescence terbium chelate
Sueda, S; Yuan, JL; Matsumoto, K
刊名BIOCONJUGATE CHEMISTRY
2002-03-01
DOI10.1021/bc010049+
13期:2页:200-205
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FLUORESCENCE ENERGY-TRANSFER ; DIKETONATE-EUROPIUM CHELATE ; LABEL ; PROBES
英文摘要Homogeneous DNA hybridization assay based on the luminescence resonance energy transfer (LRET) from a new luminescence terbium chelate, N,N,N-1,N-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (BPTA)-Tb3+ (lambda(ex) = 325 nm and lambda(em) = 545 nm) to an organic dye, Cy3 (A,. = 548 nm and A,. = 565 nm), has been developed. In the system, two DNA probes whose sequences are complementary to the two different consecutive sequences of a target DNA are used; one of the probes is labeled with the Tb3+ chelate at the T-end, and the other is with Cy3 at the 5'-end. Labeling of the Tb3+ chelate is accomplished via the linkage of a biotin-labeled DNA probe with the Tb3+ chelate-labeled streptavidin. Strong sensitized emission of Cy3 was observed upon excitation of the Tb3+ chelate at 325 run, when the two probe DNAs were hybridized with the target DNA. The sensitivity of the assay was very high compared with those of the previous homogeneous-format assays using the conventional organic dyes; the detection limit of the present assay is about 30 pM of the target DNA strand.
语种英语
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WOS记录号WOS:000174592600007
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被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/91437
专题中国科学院大连化学物理研究所
作者单位1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China
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Sueda, S,Yuan, JL,Matsumoto, K. A homogeneous DNA hybridization system by using a new luminescence terbium chelate[J]. BIOCONJUGATE CHEMISTRY,2002,13(2):200-205.
APA Sueda, S,Yuan, JL,&Matsumoto, K.(2002).A homogeneous DNA hybridization system by using a new luminescence terbium chelate.BIOCONJUGATE CHEMISTRY,13(2),200-205.
MLA Sueda, S,et al."A homogeneous DNA hybridization system by using a new luminescence terbium chelate".BIOCONJUGATE CHEMISTRY 13.2(2002):200-205.
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