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题名: A homogeneous DNA hybridization system by using a new luminescence terbium chelate
作者: Sueda, S;  Yuan, JL;  Matsumoto, K
刊名: BIOCONJUGATE CHEMISTRY
发表日期: 2002-03-01
DOI: 10.1021/bc010049+
卷: 13, 期:2, 页:200-205
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Multidisciplinary ;  Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Homogeneous DNA hybridization assay based on the luminescence resonance energy transfer (LRET) from a new luminescence terbium chelate, N,N,N-1,N-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (BPTA)-Tb3+ (lambda(ex) = 325 nm and lambda(em) = 545 nm) to an organic dye, Cy3 (A,. = 548 nm and A,. = 565 nm), has been developed. In the system, two DNA probes whose sequences are complementary to the two different consecutive sequences of a target DNA are used; one of the probes is labeled with the Tb3+ chelate at the T-end, and the other is with Cy3 at the 5'-end. Labeling of the Tb3+ chelate is accomplished via the linkage of a biotin-labeled DNA probe with the Tb3+ chelate-labeled streptavidin. Strong sensitized emission of Cy3 was observed upon excitation of the Tb3+ chelate at 325 run, when the two probe DNAs were hybridized with the target DNA. The sensitivity of the assay was very high compared with those of the previous homogeneous-format assays using the conventional organic dyes; the detection limit of the present assay is about 30 pM of the target DNA strand.
关键词[WOS]: FLUORESCENCE ENERGY-TRANSFER ;  DIKETONATE-EUROPIUM CHELATE ;  LABEL ;  PROBES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000174592600007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/91437
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Sueda, S,Yuan, JL,Matsumoto, K. A homogeneous DNA hybridization system by using a new luminescence terbium chelate[J]. BIOCONJUGATE CHEMISTRY,2002,13(2):200-205.
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