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题名: Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay
作者: Yuan, JL;  Wang, GL;  Majima, K;  Matsumoto, K
刊名: ANALYTICAL CHEMISTRY
发表日期: 2001-04-15
DOI: 10.1021/ac0013305
卷: 73, 期:8, 页:1869-1876
收录类别: SCI
文章类型: Article
部门归属: 1001
项目归属: 101
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A new nonadentate ligand, N, N, N-1, N-1-[2,6-bis(3'-aminomethyl-1 1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (BPTA) for a Tb3+ fluorescent complex was synthesized. The Tb3+ complex is strongly fluorescent, having a large fluorescence quantum yield of 1.00 and very long fluorescence lifetime of 2.681 ms in 0.05 M berate buffer of pH 9.1. Streptavidin (SA) was labeled with SPTA by using its succinimidyl monoester, and the BPTA-Tb3+-labeled SA was used in sandwich-type time-resolved fluoroimmunoassay (TR-FIA) of alpha -fetoprotein (AFP) and carcinoembryonic antigen (CEA) in human sera. The Tb3+-labeled SA was also used in competitive type TR-FIA of bensulfuron- methyl (BSM) in water. The detection limits of these assays are 42 pg/mL for AFP, 70 pg/mL for CEA, and 0.4 ng/mL for BSM. In addition, a new simultaneous measurement method for AFP and CEA in a human serum sample was developed by using 4,4'-bis(1 " ,1 " ,1 " ,2 " ,2 " ,3 " ,3 " -heptafluoro-4 " ,6 " -hexanedion-6 " -yl)chlorosulfo-o-terphenyl ((BHHCT)-Eu3+-labeled anti-AFP antibody, biotinylated anti-CEA antibody, and BPTA-Tb3+-labeled SA. The concentrations of AFP and CEA in 39 human serum samples were determined, and the results were compared with those of the independently determined AFP and CEA by TR-FIA with a single-label method. A good correlation was obtained with the correlation coefficients of 0.991 for AFP and 0.994 for CEA.
关键词[WOS]: HUMAN CHORIONIC-GONADOTROPIN ;  DIKETONATE-EUROPIUM CHELATE ;  FREE BETA-SUBUNIT ;  ALPHA-FETOPROTEIN ;  IMMUNOFLUOROMETRIC ASSAYS ;  LUMINESCENCE PROPERTIES ;  ENERGY-TRANSFER ;  LABEL ;  IMMUNOASSAY ;  PROBES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000168156600030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/91795
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Waseda Univ, Japan Sci & Technol Corp, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Yuan, JL,Wang, GL,Majima, K,et al. Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay[J]. ANALYTICAL CHEMISTRY,2001,73(8):1869-1876.
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