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Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay
Yuan, JL; Wang, GL; Majima, K; Matsumoto, K
Source PublicationANALYTICAL CHEMISTRY
2001-04-15
DOI10.1021/ac0013305
Volume73Issue:8Pages:1869-1876
Indexed BySCI
SubtypeArticle
Department1001
Funding Project101
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHUMAN CHORIONIC-GONADOTROPIN ; DIKETONATE-EUROPIUM CHELATE ; FREE BETA-SUBUNIT ; ALPHA-FETOPROTEIN ; IMMUNOFLUOROMETRIC ASSAYS ; LUMINESCENCE PROPERTIES ; ENERGY-TRANSFER ; LABEL ; IMMUNOASSAY ; PROBES
AbstractA new nonadentate ligand, N, N, N-1, N-1-[2,6-bis(3'-aminomethyl-1 1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (BPTA) for a Tb3+ fluorescent complex was synthesized. The Tb3+ complex is strongly fluorescent, having a large fluorescence quantum yield of 1.00 and very long fluorescence lifetime of 2.681 ms in 0.05 M berate buffer of pH 9.1. Streptavidin (SA) was labeled with SPTA by using its succinimidyl monoester, and the BPTA-Tb3+-labeled SA was used in sandwich-type time-resolved fluoroimmunoassay (TR-FIA) of alpha -fetoprotein (AFP) and carcinoembryonic antigen (CEA) in human sera. The Tb3+-labeled SA was also used in competitive type TR-FIA of bensulfuron- methyl (BSM) in water. The detection limits of these assays are 42 pg/mL for AFP, 70 pg/mL for CEA, and 0.4 ng/mL for BSM. In addition, a new simultaneous measurement method for AFP and CEA in a human serum sample was developed by using 4,4'-bis(1 " ,1 " ,1 " ,2 " ,2 " ,3 " ,3 " -heptafluoro-4 " ,6 " -hexanedion-6 " -yl)chlorosulfo-o-terphenyl ((BHHCT)-Eu3+-labeled anti-AFP antibody, biotinylated anti-CEA antibody, and BPTA-Tb3+-labeled SA. The concentrations of AFP and CEA in 39 human serum samples were determined, and the results were compared with those of the independently determined AFP and CEA by TR-FIA with a single-label method. A good correlation was obtained with the correlation coefficients of 0.991 for AFP and 0.994 for CEA.
Language英语
URL查看原文
WOS IDWOS:000168156600030
Citation statistics
Cited Times:203[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/91795
Collection中国科学院大连化学物理研究所
Affiliation1.Waseda Univ, Japan Sci & Technol Corp, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Yuan, JL,Wang, GL,Majima, K,et al. Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay[J]. ANALYTICAL CHEMISTRY,2001,73(8):1869-1876.
APA Yuan, JL,Wang, GL,Majima, K,&Matsumoto, K.(2001).Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay.ANALYTICAL CHEMISTRY,73(8),1869-1876.
MLA Yuan, JL,et al."Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay".ANALYTICAL CHEMISTRY 73.8(2001):1869-1876.
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