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题名: Homogeneous time-resolved fluoroimmunoassay of bensulfuron-methyl by using terbium fluorescence energy transfer
作者: Wang, GL;  Yuan, JL;  Matsumoto, K;  Hu, ZD
关键词: homogeneous immunoassay ;  fluorescence energy transfer ;  terbium ;  bensulfuron-methyl
刊名: TALANTA
发表日期: 2001-12-24
卷: 55, 期:6, 页:1119-1125
收录类别: SCI
文章类型: Article
部门归属: 1003
项目归属: 101
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A sensitive homogenous time-resolved fluoroimmunoassay (TR-FIA) method for bensulfuron-methyl (BSM) based on fluorescence resonance energy transfer (FRET) from a Tb3+ fluorescent chelate with N,N,N',N'-[2,6-bis(3'-aminomethyl-1'-pyrazoly)-4-phenylpyridine] tetrakis(acetic acid) (BPTA-Tb3+) to organic dye, Cy3 or Cy3.5 has been developed. New method combined the use of BPTA-Tb3+ labeled streptavidin, Cy3 or Cy3.5 labeled anti-BSM monoclonal antibody and biotinylated BSM-BSA conjugate (BSA is bovine serum albumin) for competitive-type immunoassay. After BPTA-Tb3+ labeled streptavidin was reacted with a competitive immune reaction solution containing biotinylated BSM-BSA, BSM sample and Cy3 or Cy3.5 labeled anti-BSM monoclonal antibody, the sensitized and long-lived emission of Cy3 or Cy3.5 derived from FRET was measured, and thus the concentration of BSM in sample was calculated. The present method has the advantages of rapidity, simplicity and high sensitivity since the B/F (bound reagent/free reagent) separation steps and the solid-phase carrier are not necessary. The method gives the detection limit of 2.10 ng ml(-1). The coefficient variations of the method are less than 1.5% and the recoveries are in the range of 95-105% for BSM water sample measurement. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: EUROPIUM CHELATE ;  IMMUNOASSAYS ;  LABELS ;  ASSAYS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000173038600012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/91799
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
2.Lanzhou Univ, Dept Chem, Lanzhou 730001, Peoples R China
3.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan

Recommended Citation:
Wang, GL,Yuan, JL,Matsumoto, K,et al. Homogeneous time-resolved fluoroimmunoassay of bensulfuron-methyl by using terbium fluorescence energy transfer[J]. TALANTA,2001,55(6):1119-1125.
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