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题名: Modeling of protein adsorption in membrane affinity chromatography
作者: Hao, WQ;  Chen, ZA;  Wang, JD;  Liu, XL
关键词: membrane affinity chromatography ;  ideal adsorption model ;  equilibrium-dispersive model ;  cellulose diacetate/polyethyleneimine blend membranes ;  frontal analysis ;  bovine serum albumin
刊名: ANALYTICAL LETTERS
发表日期: 2004
DOI: 10.1080/AL-120035901
卷: 37, 期:7, 页:1319-1338
收录类别: SCI
文章类型: Article
项目归属: 102
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: For the design of affinity membranes, protein adsorption in membrane affinity chromatography (MAC) was studied by frontal analysis. According to fast mass transfer, small thickness of affinity membranes and high affinity between the protein and the ligand, an ideal adsorption (IA) model was proposed for MAC and was used together with equilibrium-dispersive (E-D) model to describe the adsorption of bovine serum albumin (BSA) onto cellulose diacetate/polyethyleneimine (CA/PEI) blend membranes with and without Cu2+ chelating. E-D model was found to better describe the initial region of experimental breakthrough curves. The influence of axial dispersion was revealed and it showed the importance of design of the module to homogenously distribute feed solution. IA model was found to be better for the whole experimental breakthrough curve. According to it, the capacity of affinity membranes and the specificity of the interaction are of equal importance for the design of affinity membranes. An optimum feed concentration was also found in the operation of MAC. The discrepancy between experimental optimum feed concentrations and predicted ones from IA model may be due to the ignorance of some experimental effects such as axial dispersion.
关键词[WOS]: SORPTION KINETICS ;  BIOSEPARATIONS ;  SEPARATION ;  BREAKTHROUGH ;  COLUMNS ;  DESIGN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000221935400005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/91835
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Hao, WQ,Chen, ZA,Wang, JD,et al. Modeling of protein adsorption in membrane affinity chromatography[J]. ANALYTICAL LETTERS,2004,37(7):1319-1338.
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