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Modeling of protein adsorption in membrane affinity chromatography
Hao, WQ; Chen, ZA; Wang, JD; Liu, XL
KeywordMembrane Affinity Chromatography Ideal Adsorption Model Equilibrium-dispersive Model Cellulose Diacetate/polyethyleneimine Blend Membranes Frontal Analysis Bovine Serum Albumin
Source PublicationANALYTICAL LETTERS
2004
DOI10.1080/AL-120035901
Volume37Issue:7Pages:1319-1338
Indexed BySCI
SubtypeArticle
Funding Project102
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSORPTION KINETICS ; BIOSEPARATIONS ; SEPARATION ; BREAKTHROUGH ; COLUMNS ; DESIGN
AbstractFor the design of affinity membranes, protein adsorption in membrane affinity chromatography (MAC) was studied by frontal analysis. According to fast mass transfer, small thickness of affinity membranes and high affinity between the protein and the ligand, an ideal adsorption (IA) model was proposed for MAC and was used together with equilibrium-dispersive (E-D) model to describe the adsorption of bovine serum albumin (BSA) onto cellulose diacetate/polyethyleneimine (CA/PEI) blend membranes with and without Cu2+ chelating. E-D model was found to better describe the initial region of experimental breakthrough curves. The influence of axial dispersion was revealed and it showed the importance of design of the module to homogenously distribute feed solution. IA model was found to be better for the whole experimental breakthrough curve. According to it, the capacity of affinity membranes and the specificity of the interaction are of equal importance for the design of affinity membranes. An optimum feed concentration was also found in the operation of MAC. The discrepancy between experimental optimum feed concentrations and predicted ones from IA model may be due to the ignorance of some experimental effects such as axial dispersion.
Language英语
URL查看原文
WOS IDWOS:000221935400005
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/91835
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Hao, WQ,Chen, ZA,Wang, JD,et al. Modeling of protein adsorption in membrane affinity chromatography[J]. ANALYTICAL LETTERS,2004,37(7):1319-1338.
APA Hao, WQ,Chen, ZA,Wang, JD,&Liu, XL.(2004).Modeling of protein adsorption in membrane affinity chromatography.ANALYTICAL LETTERS,37(7),1319-1338.
MLA Hao, WQ,et al."Modeling of protein adsorption in membrane affinity chromatography".ANALYTICAL LETTERS 37.7(2004):1319-1338.
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