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题名: Highly sensitive determination of the methylated p16 gene in cancer patients by microchip electrophoresis
作者: Zhou, XM;  Shao, SJ;  Xu, GD;  Zhong, RT;  Liu, DY;  Tang, JW;  Gao, YN;  Cheng, SJ;  Lin, BC
通讯作者: 林炳承
关键词: microchip electrophoresis ;  DNA methylation ;  cancer diagnosis
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2005-02-25
DOI: 10.1016/j.jchromb.2004.11.045
卷: 816, 期:1-2, 页:145-151
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The p16 tumor suppressor gene is inactivated by promoter region hypermethylation in many types of tumor. Recent studies showed that aberrant methylation of the p16 gene is an early event in many tumors, especially in lung cancer, and may constitute a new biomarker for early detection and monitoring of prevention trials. We detected tumor-associated aberrant hypermethylation of the p16 gene in plasma and tissue DNA from 153 specimens using a modified semi-nested methylation-specific PCR (MSP) combining plastic microchip electrophoresis or slab gel electrophoresis, respectively. Specimens were from 79 lung cancer patients, 15 abdominal tumor patients, 30 positive controls and 30 negative controls. The results showed that the positive rate obtained by microchip electrophoresis was more than 26.6% higher and the same speciticity was kept when compared with slab gel electrophoresis. The microchip electrophoresis can rapidly and accurately analyze the PCR products of methylated DNA and obviously improve the positive rate of diagnosis of cancer patients when compared with gel electrophoresis. This method with the high assay sensitivity might be used for detection of methylation of p16 gene and even to facilitate early diagnosis of cancer patients. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  LUNG-CANCER ;  PROMOTER HYPERMETHYLATION ;  DNA ;  SERUM ;  PLASMA ;  CHIP
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000226879800019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92145
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China
3.Dalian Med Univ, Dalian 116023, Peoples R China
4.Peking Union Med Coll, Canc Inst Hosp, Dept Chem Etiol & Carcinogenesis, Beijing 100021, Peoples R China
5.Chinese Acad Med Sci, Beijing 100021, Peoples R China

Recommended Citation:
Zhou, XM,Shao, SJ,Xu, GD,et al. Highly sensitive determination of the methylated p16 gene in cancer patients by microchip electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,816(1-2):145-151.
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