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Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA
Zhou, XM; Dai, ZP; Liu, X; Luo, Y; Wang, H; Lin, BC; Lin BC(林炳承); Lin BC(林炳承)
KeywordDna Separation Electrophoretic Microchip Fabrication Modification
Source PublicationJOURNAL OF SEPARATION SCIENCE
2005-02-01
ISSN1615-9306
DOI10.1002/jssc.200401920
Volume28Issue:3Pages:225-233
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCAPILLARY-ELECTROPHORESIS ; MICROFLUIDIC DEVICES ; FABRICATION ; POLY(DIMETHYLSILOXANE) ; MOLECULES ; FRAGMENTS ; FLOW
AbstractInexpensive and permanently modified poly(methyl methacrylate)(PMMA) microchips were fabricated by an injection-molding process. A novel sealing method for plastic microchips at room temperature was introduced. Run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values between 1-3% for the run-to-run and less than 2.1% for the chip-to-chip comparisons. Acrylonitrile-butadiene-styrene (ABS) was used as an additive in PMMA substrates. The proportions of PMMA and ABS were optimized. ABS may be considered as a modifier, which obviously improved some characteristics of the microchip, such as the hydrophilicity and the electro-osmotic flow (EOF). The detection limit of Rhodamine 6G dye for the modified microchip on the home-made microchip analyzer showed a dramatic 100-fold improvement over that for the unmodified PMMA chip. A detection limit of the order of 10(-20) mole has been achieved for each injected phiX-174/HaeIII DNA fragment with the baseline separation between 271 and 281 bp, and fast separation of 11 DNA restriction fragments within 180 seconds. Analysis of a PCR product from the tobacco ACT gene was performed on the modified microchip as an application example.
Language英语
URL查看原文
WOS IDWOS:000227397400002
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92149
Collection中国科学院大连化学物理研究所
Corresponding AuthorLin BC(林炳承); Lin BC(林炳承)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China
Recommended Citation
GB/T 7714
Zhou, XM,Dai, ZP,Liu, X,et al. Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(3):225-233.
APA Zhou, XM.,Dai, ZP.,Liu, X.,Luo, Y.,Wang, H.,...&林炳承.(2005).Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA.JOURNAL OF SEPARATION SCIENCE,28(3),225-233.
MLA Zhou, XM,et al."Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA".JOURNAL OF SEPARATION SCIENCE 28.3(2005):225-233.
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