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题名: Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA
作者: Zhou, XM;  Dai, ZP;  Liu, X;  Luo, Y;  Wang, H;  Lin, BC
通讯作者: 林炳承
关键词: DNA separation ;  electrophoretic microchip ;  fabrication ;  modification
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2005-02-01
DOI: 10.1002/jssc.200401920
卷: 28, 期:3, 页:225-233
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Inexpensive and permanently modified poly(methyl methacrylate)(PMMA) microchips were fabricated by an injection-molding process. A novel sealing method for plastic microchips at room temperature was introduced. Run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values between 1-3% for the run-to-run and less than 2.1% for the chip-to-chip comparisons. Acrylonitrile-butadiene-styrene (ABS) was used as an additive in PMMA substrates. The proportions of PMMA and ABS were optimized. ABS may be considered as a modifier, which obviously improved some characteristics of the microchip, such as the hydrophilicity and the electro-osmotic flow (EOF). The detection limit of Rhodamine 6G dye for the modified microchip on the home-made microchip analyzer showed a dramatic 100-fold improvement over that for the unmodified PMMA chip. A detection limit of the order of 10(-20) mole has been achieved for each injected phiX-174/HaeIII DNA fragment with the baseline separation between 271 and 281 bp, and fast separation of 11 DNA restriction fragments within 180 seconds. Analysis of a PCR product from the tobacco ACT gene was performed on the modified microchip as an application example.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MICROFLUIDIC DEVICES ;  FABRICATION ;  POLY(DIMETHYLSILOXANE) ;  MOLECULES ;  FRAGMENTS ;  FLOW
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000227397400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92149
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China

Recommended Citation:
Zhou, XM,Dai, ZP,Liu, X,et al. Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(3):225-233.
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