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题名: Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification
作者: Jin, WH;  Dai, J;  Li, SJ;  Xia, QC;  Zou, HF;  Zeng, R
通讯作者: 邹汉法 ;  曾嵘
关键词: proteomics ;  human plasma ;  mass spectrometry ;  two-dimensional liquid chromatography ;  protein fractionation
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2005-03-01
DOI: 10.1021/pr049761h
卷: 4, 期:2, 页:613-619
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A resurgence of interest in the human plasma proteome has occurred in recent years because it holds great promise of revolution in disease diagnosis and therapeutic monitoring. As one of the most powerful separation techniques, multidimensional liquid chromatography has attracted extensive attention, but most published works have focused on the fractionation of tryptic peptides. In this study, proteins from human plasma were prefractionated by online sequential strong cation exchange chromatography and reversed-phase chromatography. The resulting 30 samples were individually digested by trypsin, and analyzed by capillary reversed-phase liquid chromatography coupled with linear ion trap mass spectrometry. After meeting stringent criteria, a total of 1292 distinct proteins were successfully identified in our work, among which, some proteins known to be present in serum in < 10 ng/mL were detected. Compared with other works in published literatures, this analysis offered a more full-scale list of the plasma proteome. Considering our strategy allows high throughput of protein identification in serum, the prefractionation of proteins before MS analysis is a simple and effective method to facilitate human plasma proteome research.
关键词[WOS]: RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS ;  HUMAN SERUM PROTEOME ;  HEPATOCELLULAR-CARCINOMA ;  CANCER-DIAGNOSIS ;  OVARIAN-CANCER ;  PROTEINS ;  BIOMARKERS ;  POPULATION ;  SYSTEM ;  GELS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000228421900049
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92177
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Proteome Anal, Inst Biochem & Cell Biol,Key Lab Proteom, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Jin, WH,Dai, J,Li, SJ,et al. Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification[J]. JOURNAL OF PROTEOME RESEARCH,2005,4(2):613-619.
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