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题名: Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins
作者: Yang, C;  Zhang, WB;  Zhang, J;  Duan, JC;  Zhang, YK
通讯作者: 张玉奎
关键词: isoelectric focusing ;  pH gradient ;  extremely acidic and basic proteins
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2005
DOI: 10.1002/jssc.200401793
卷: 28, 期:1, 页:78-86
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A new set-up was constructed for capillary isoelectric focusing (CIEF) involving a sampling capillary as a bypass fixed to the separation capillary. Sample solutions were subjected to a previously established pH gradient from the sample capillary. Besides performing conventional CIEF, the separation of ampholytic compounds with isoelectric points (p/s) beyond the pH gradient was carried out on this system. This method was termed as pH gradient driven electrophoresis (PGDE) and the basic mathematical expressions were derived to express the dynamic fundamentals. Proteins such as lysozyme, cytochrome C, and pepsin with p/s higher than 10 or below 3 were separated in a pH gradient provided by Pharmalyte (pH 3-10). Finally, this protocol convincingly exhibited its potential in the separation of a solution of chicken egg white.
关键词[WOS]: HIGH-PERFORMANCE ELECTROPHORESIS ;  EQUILIBRIUM-GRADIENT METHODS ;  ZONE ELECTROPHORESIS ;  ELECTROKINETIC CHROMATOGRAPHY ;  EXPERIMENTAL VALIDATION ;  COMPUTER-SIMULATION ;  ISOTACHOPHORESIS ;  SYSTEM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000226463300010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92185
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Yang, C,Zhang, WB,Zhang, J,et al. Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(1):78-86.
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