DICP OpenIR
Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins
Yang, C; Zhang, WB; Zhang, J; Duan, JC; Zhang, YK; Zhang YK(张玉奎); Zhang YK(张玉奎)
关键词Isoelectric Focusing Ph Gradient Extremely Acidic And Basic Proteins
刊名JOURNAL OF SEPARATION SCIENCE
2005
ISSN1615-9306
DOI10.1002/jssc.200401793
28期:1页:78-86
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HIGH-PERFORMANCE ELECTROPHORESIS ; EQUILIBRIUM-GRADIENT METHODS ; ZONE ELECTROPHORESIS ; ELECTROKINETIC CHROMATOGRAPHY ; EXPERIMENTAL VALIDATION ; COMPUTER-SIMULATION ; ISOTACHOPHORESIS ; SYSTEM
英文摘要A new set-up was constructed for capillary isoelectric focusing (CIEF) involving a sampling capillary as a bypass fixed to the separation capillary. Sample solutions were subjected to a previously established pH gradient from the sample capillary. Besides performing conventional CIEF, the separation of ampholytic compounds with isoelectric points (p/s) beyond the pH gradient was carried out on this system. This method was termed as pH gradient driven electrophoresis (PGDE) and the basic mathematical expressions were derived to express the dynamic fundamentals. Proteins such as lysozyme, cytochrome C, and pepsin with p/s higher than 10 or below 3 were separated in a pH gradient provided by Pharmalyte (pH 3-10). Finally, this protocol convincingly exhibited its potential in the separation of a solution of chicken egg white.
语种英语
原文出处查看原文
WOS记录号WOS:000226463300010
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/92185
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎); Zhang YK(张玉奎)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Yang, C,Zhang, WB,Zhang, J,et al. Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(1):78-86.
APA Yang, C.,Zhang, WB.,Zhang, J.,Duan, JC.,Zhang, YK.,...&张玉奎.(2005).Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins.JOURNAL OF SEPARATION SCIENCE,28(1),78-86.
MLA Yang, C,et al."Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins".JOURNAL OF SEPARATION SCIENCE 28.1(2005):78-86.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2005W6D0166N45.pdf(330KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, C]的文章
[Zhang, WB]的文章
[Zhang, J]的文章
百度学术
百度学术中相似的文章
[Yang, C]的文章
[Zhang, WB]的文章
[Zhang, J]的文章
必应学术
必应学术中相似的文章
[Yang, C]的文章
[Zhang, WB]的文章
[Zhang, J]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。