DICP OpenIR
Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins
Yang, C; Zhang, WB; Zhang, J; Duan, JC; Zhang, YK; Zhang YK(张玉奎); Zhang YK(张玉奎)
KeywordIsoelectric Focusing Ph Gradient Extremely Acidic And Basic Proteins
Source PublicationJOURNAL OF SEPARATION SCIENCE
2005
ISSN1615-9306
DOI10.1002/jssc.200401793
Volume28Issue:1Pages:78-86
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHIGH-PERFORMANCE ELECTROPHORESIS ; EQUILIBRIUM-GRADIENT METHODS ; ZONE ELECTROPHORESIS ; ELECTROKINETIC CHROMATOGRAPHY ; EXPERIMENTAL VALIDATION ; COMPUTER-SIMULATION ; ISOTACHOPHORESIS ; SYSTEM
AbstractA new set-up was constructed for capillary isoelectric focusing (CIEF) involving a sampling capillary as a bypass fixed to the separation capillary. Sample solutions were subjected to a previously established pH gradient from the sample capillary. Besides performing conventional CIEF, the separation of ampholytic compounds with isoelectric points (p/s) beyond the pH gradient was carried out on this system. This method was termed as pH gradient driven electrophoresis (PGDE) and the basic mathematical expressions were derived to express the dynamic fundamentals. Proteins such as lysozyme, cytochrome C, and pepsin with p/s higher than 10 or below 3 were separated in a pH gradient provided by Pharmalyte (pH 3-10). Finally, this protocol convincingly exhibited its potential in the separation of a solution of chicken egg white.
Language英语
URL查看原文
WOS IDWOS:000226463300010
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92185
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang YK(张玉奎); Zhang YK(张玉奎)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, C,Zhang, WB,Zhang, J,et al. Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(1):78-86.
APA Yang, C.,Zhang, WB.,Zhang, J.,Duan, JC.,Zhang, YK.,...&张玉奎.(2005).Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins.JOURNAL OF SEPARATION SCIENCE,28(1),78-86.
MLA Yang, C,et al."Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins".JOURNAL OF SEPARATION SCIENCE 28.1(2005):78-86.
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