中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry
作者: Liang, Z;  Zhang, LH;  Duan, JC;  Yan, C;  Zhang, WB;  Zhang, YK
通讯作者: 张玉奎
关键词: on-line concentration ;  pressurized capillary electrochromatography-mass spectrometry ;  protein
刊名: ELECTROPHORESIS
发表日期: 2005-04-01
DOI: 10.1002/elps.200406200
卷: 26, 期:7-8, 页:1398-1405
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Pressurized capillary electrochromatography (pCEC) and electrospray ionization-mass spectrometry (ESI-MS) have been hyphenated for protein analysis. Taken cytochrome c, lysozyme, and insulin as samples, the limits of detection (LODs) for absolute concentrations are 10(-11) mol (signal-to-noise ratio S/N = 3) with relative standard deviations (RSDs) of retention time and peak area, respectively, of less than 1.7% and 4.8%. In order to improve the detection sensitivity, on-line concentration by field-enhanced sample-stacking effect and chromatographic zone-sharpening effect has been developed, and parameters affecting separation and detection, such as pH and electrolyte concentration in the mobile phase, separation voltage, as well as enrichment voltage and time, have been studied systematically. Under the optimized conditions, the LODs of the three proteins could be decreased up to 100-fold. In addition, the feasibility of such techniques has been further demonstrated by the analysis of modified insulins at a concentration of 20 mu g/mL.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  PRECONCENTRATION ;  SENSITIVITY ;  PHASE ;  FLOW ;  MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000228784000014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92235
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2005LN1ZF5R660.pdf(483KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA USA

Recommended Citation:
Liang, Z,Zhang, LH,Duan, JC,et al. On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry[J]. ELECTROPHORESIS,2005,26(7-8):1398-1405.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Liang, Z]'s Articles
 [Zhang, LH]'s Articles
 [Duan, JC]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Liang, Z]‘s Articles
 [Zhang, LH]‘s Articles
 [Duan, JC]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2005LN1ZF5R660.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace