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On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry
Liang, Z; Zhang, LH; Duan, JC; Yan, C; Zhang, WB; Zhang, YK; Zhang YK(张玉奎); Zhang YK(张玉奎)
关键词On-line Concentration Pressurized Capillary Electrochromatography-mass Spectrometry Protein
刊名ELECTROPHORESIS
2005-04-01
ISSN0173-0835
DOI10.1002/elps.200406200
26期:7-8页:1398-1405
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; PRECONCENTRATION ; SENSITIVITY ; PHASE ; FLOW ; MS
英文摘要Pressurized capillary electrochromatography (pCEC) and electrospray ionization-mass spectrometry (ESI-MS) have been hyphenated for protein analysis. Taken cytochrome c, lysozyme, and insulin as samples, the limits of detection (LODs) for absolute concentrations are 10(-11) mol (signal-to-noise ratio S/N = 3) with relative standard deviations (RSDs) of retention time and peak area, respectively, of less than 1.7% and 4.8%. In order to improve the detection sensitivity, on-line concentration by field-enhanced sample-stacking effect and chromatographic zone-sharpening effect has been developed, and parameters affecting separation and detection, such as pH and electrolyte concentration in the mobile phase, separation voltage, as well as enrichment voltage and time, have been studied systematically. Under the optimized conditions, the LODs of the three proteins could be decreased up to 100-fold. In addition, the feasibility of such techniques has been further demonstrated by the analysis of modified insulins at a concentration of 20 mu g/mL.
语种英语
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WOS记录号WOS:000228784000014
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/92235
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎); Zhang YK(张玉奎)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA USA
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GB/T 7714
Liang, Z,Zhang, LH,Duan, JC,et al. On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry[J]. ELECTROPHORESIS,2005,26(7-8):1398-1405.
APA Liang, Z.,Zhang, LH.,Duan, JC.,Yan, C.,Zhang, WB.,...&张玉奎.(2005).On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry.ELECTROPHORESIS,26(7-8),1398-1405.
MLA Liang, Z,et al."On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry".ELECTROPHORESIS 26.7-8(2005):1398-1405.
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