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题名: On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis
作者: Zhang, LY;  Zhang, LH;  Zhang, WB;  Zhang, YK
通讯作者: 张玉奎
关键词: capillary electrophoresis ;  Cu(II)-immobilized metal affinity chromatography ;  monolithic column ;  on-line concentration ;  peptide ;  protein
刊名: ELECTROPHORESIS
发表日期: 2005-06-01
DOI: 10.1002/elps.200410377
卷: 26, 期:11, 页:2172-2178
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An iminodiacetic acid (IDA)-type adsorbent is prepared at the one end of a capillary by covalently bonding IDA to the monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate). Cu(II) is later introduced to the support via the interaction with IDA. By this means, polymer monolithic immobilized metal affinity chromatography (IMAC) materials are prepared. With such a column, IMAC for on-line concentration and capillary electrophoresis (CE) for the subsequent analysis are hyphenated for the analysis of peptides and proteins. The reproducibility of such a column has been proved good with relative standard deviations (RSDs) of dead time of less than 5% for injection-to-injection and 12% for column-to-column (n = 3). Through application on the analysis of standard peptides and real protein samples, such a technique has shown promising in proteome study.
关键词[WOS]: MICELLAR ELECTROKINETIC CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  FLUORESCENCE DETECTION ;  ELECTROCHROMATOGRAPHY ;  PHASE ;  EXTRACTION ;  SEPARATION ;  EXCHANGE ;  COLUMNS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000229901500013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92237
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, LY,Zhang, LH,Zhang, WB,et al. On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis[J]. ELECTROPHORESIS,2005,26(11):2172-2178.
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