DICP OpenIR
On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis
Zhang, LY; Zhang, LH; Zhang, WB; Zhang, YK; Zhang YK(张玉奎); Zhang YK(张玉奎)
KeywordCapillary Electrophoresis Cu(Ii)-immobilized Metal Affinity Chromatography Monolithic Column On-line Concentration Peptide Protein
Source PublicationELECTROPHORESIS
2005-06-01
ISSN0173-0835
DOI10.1002/elps.200410377
Volume26Issue:11Pages:2172-2178
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMICELLAR ELECTROKINETIC CHROMATOGRAPHY ; MASS-SPECTROMETRY ; FLUORESCENCE DETECTION ; ELECTROCHROMATOGRAPHY ; PHASE ; EXTRACTION ; SEPARATION ; EXCHANGE ; COLUMNS
AbstractAn iminodiacetic acid (IDA)-type adsorbent is prepared at the one end of a capillary by covalently bonding IDA to the monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate). Cu(II) is later introduced to the support via the interaction with IDA. By this means, polymer monolithic immobilized metal affinity chromatography (IMAC) materials are prepared. With such a column, IMAC for on-line concentration and capillary electrophoresis (CE) for the subsequent analysis are hyphenated for the analysis of peptides and proteins. The reproducibility of such a column has been proved good with relative standard deviations (RSDs) of dead time of less than 5% for injection-to-injection and 12% for column-to-column (n = 3). Through application on the analysis of standard peptides and real protein samples, such a technique has shown promising in proteome study.
Language英语
URL查看原文
WOS IDWOS:000229901500013
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92237
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang YK(张玉奎); Zhang YK(张玉奎)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, LY,Zhang, LH,Zhang, WB,et al. On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis[J]. ELECTROPHORESIS,2005,26(11):2172-2178.
APA Zhang, LY,Zhang, LH,Zhang, WB,Zhang, YK,张玉奎,&张玉奎.(2005).On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis.ELECTROPHORESIS,26(11),2172-2178.
MLA Zhang, LY,et al."On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis".ELECTROPHORESIS 26.11(2005):2172-2178.
Files in This Item:
File Name/Size DocType Version Access License
2005976RQ7K521.pdf(271KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, LY]'s Articles
[Zhang, LH]'s Articles
[Zhang, WB]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, LY]'s Articles
[Zhang, LH]'s Articles
[Zhang, WB]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, LY]'s Articles
[Zhang, LH]'s Articles
[Zhang, WB]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.