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On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis
Zhang, LY; Zhang, LH; Zhang, WB; Zhang, YK; Zhang YK(张玉奎); Zhang YK(张玉奎)
关键词Capillary Electrophoresis Cu(Ii)-immobilized Metal Affinity Chromatography Monolithic Column On-line Concentration Peptide Protein
刊名ELECTROPHORESIS
2005-06-01
ISSN0173-0835
DOI10.1002/elps.200410377
26期:11页:2172-2178
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; MASS-SPECTROMETRY ; FLUORESCENCE DETECTION ; ELECTROCHROMATOGRAPHY ; PHASE ; EXTRACTION ; SEPARATION ; EXCHANGE ; COLUMNS
英文摘要An iminodiacetic acid (IDA)-type adsorbent is prepared at the one end of a capillary by covalently bonding IDA to the monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate). Cu(II) is later introduced to the support via the interaction with IDA. By this means, polymer monolithic immobilized metal affinity chromatography (IMAC) materials are prepared. With such a column, IMAC for on-line concentration and capillary electrophoresis (CE) for the subsequent analysis are hyphenated for the analysis of peptides and proteins. The reproducibility of such a column has been proved good with relative standard deviations (RSDs) of dead time of less than 5% for injection-to-injection and 12% for column-to-column (n = 3). Through application on the analysis of standard peptides and real protein samples, such a technique has shown promising in proteome study.
语种英语
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WOS记录号WOS:000229901500013
引用统计
被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/92237
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎); Zhang YK(张玉奎)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Zhang, LY,Zhang, LH,Zhang, WB,et al. On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis[J]. ELECTROPHORESIS,2005,26(11):2172-2178.
APA Zhang, LY,Zhang, LH,Zhang, WB,Zhang, YK,张玉奎,&张玉奎.(2005).On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis.ELECTROPHORESIS,26(11),2172-2178.
MLA Zhang, LY,et al."On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis".ELECTROPHORESIS 26.11(2005):2172-2178.
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