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Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection
Qin, JH; Leung, FC; Fung, YS; Zhu, DR; Lin, BC; Lin BC(林炳承)
KeywordGinseng Short Tandem Repeats (Str) Microchip Electrophoresis Molecular Authentication
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2005-02-01
ISSN1618-2642
DOI10.1007/s00216-004-2889-2
Volume381Issue:4Pages:812-819
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPOLYMERASE CHAIN-REACTION ; NORTH-AMERICAN GINSENG ; TOTAL ANALYSIS SYSTEMS ; CAPILLARY-ELECTROPHORESIS ; TANDEM REPEATS ; DNA ; IDENTIFICATION ; STANDARD ; ROOTS ; PCR
AbstractGinseng is one of the most expensive Chinese herbal medicines and the effectiveness of ginseng depends strongly on its botanical sources and the use of different parts of the plants. In this study, a microchip electrophoresis method coupled with the polymerase chain reaction (PCR)-short tandem repeats (STR) technique was developed for rapid authentication of ginseng species. A low viscosity hydroxypropyl methylcellulose (HPMC) solution was used as the sieving matrix for separation of the amplified STR fragments. The allele sizing of the amplified PCR products could be detected within 240 s or less. Good reproducibility and accuracy of the fragment size were obtained with the relative standard deviation for the allele sizes less than 1.0% (n = 11). At two microsatellite loci (CT 12, CA 33), American ginseng had a different allele pattern on the electropherograms compared with that of the Oriental ginseng. Moreover, cultivated and wild American ginseng can be distinguished on the basis of allele sizing. This work establishes the feasibility of fast genetic authentication of ginseng species by use of microchip electrophoresis.
Language英语
URL查看原文
WOS IDWOS:000227450200003
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92273
Collection中国科学院大连化学物理研究所
Corresponding AuthorLin BC(林炳承)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China
3.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Qin, JH,Leung, FC,Fung, YS,et al. Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,381(4):812-819.
APA Qin, JH,Leung, FC,Fung, YS,Zhu, DR,Lin, BC,&林炳承.(2005).Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,381(4),812-819.
MLA Qin, JH,et al."Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 381.4(2005):812-819.
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