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题名: Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection
作者: Qin, JH;  Leung, FC;  Fung, YS;  Zhu, DR;  Lin, BC
通讯作者: 林炳承
关键词: ginseng ;  short tandem repeats (STR) ;  microchip ;  electrophoresis ;  molecular authentication
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2005-02-01
DOI: 10.1007/s00216-004-2889-2
卷: 381, 期:4, 页:812-819
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Ginseng is one of the most expensive Chinese herbal medicines and the effectiveness of ginseng depends strongly on its botanical sources and the use of different parts of the plants. In this study, a microchip electrophoresis method coupled with the polymerase chain reaction (PCR)-short tandem repeats (STR) technique was developed for rapid authentication of ginseng species. A low viscosity hydroxypropyl methylcellulose (HPMC) solution was used as the sieving matrix for separation of the amplified STR fragments. The allele sizing of the amplified PCR products could be detected within 240 s or less. Good reproducibility and accuracy of the fragment size were obtained with the relative standard deviation for the allele sizes less than 1.0% (n = 11). At two microsatellite loci (CT 12, CA 33), American ginseng had a different allele pattern on the electropherograms compared with that of the Oriental ginseng. Moreover, cultivated and wild American ginseng can be distinguished on the basis of allele sizing. This work establishes the feasibility of fast genetic authentication of ginseng species by use of microchip electrophoresis.
关键词[WOS]: POLYMERASE CHAIN-REACTION ;  NORTH-AMERICAN GINSENG ;  TOTAL ANALYSIS SYSTEMS ;  CAPILLARY-ELECTROPHORESIS ;  TANDEM REPEATS ;  DNA ;  IDENTIFICATION ;  STANDARD ;  ROOTS ;  PCR
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000227450200003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92273
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China
3.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Qin, JH,Leung, FC,Fung, YS,et al. Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,381(4):812-819.
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