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Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection
Qin, JH; Leung, FC; Fung, YS; Zhu, DR; Lin, BC; Lin BC(林炳承)
关键词Ginseng Short Tandem Repeats (Str) Microchip Electrophoresis Molecular Authentication
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2005-02-01
ISSN1618-2642
DOI10.1007/s00216-004-2889-2
381期:4页:812-819
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]POLYMERASE CHAIN-REACTION ; NORTH-AMERICAN GINSENG ; TOTAL ANALYSIS SYSTEMS ; CAPILLARY-ELECTROPHORESIS ; TANDEM REPEATS ; DNA ; IDENTIFICATION ; STANDARD ; ROOTS ; PCR
英文摘要Ginseng is one of the most expensive Chinese herbal medicines and the effectiveness of ginseng depends strongly on its botanical sources and the use of different parts of the plants. In this study, a microchip electrophoresis method coupled with the polymerase chain reaction (PCR)-short tandem repeats (STR) technique was developed for rapid authentication of ginseng species. A low viscosity hydroxypropyl methylcellulose (HPMC) solution was used as the sieving matrix for separation of the amplified STR fragments. The allele sizing of the amplified PCR products could be detected within 240 s or less. Good reproducibility and accuracy of the fragment size were obtained with the relative standard deviation for the allele sizes less than 1.0% (n = 11). At two microsatellite loci (CT 12, CA 33), American ginseng had a different allele pattern on the electropherograms compared with that of the Oriental ginseng. Moreover, cultivated and wild American ginseng can be distinguished on the basis of allele sizing. This work establishes the feasibility of fast genetic authentication of ginseng species by use of microchip electrophoresis.
语种英语
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WOS记录号WOS:000227450200003
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被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/92273
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China
3.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
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Qin, JH,Leung, FC,Fung, YS,et al. Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,381(4):812-819.
APA Qin, JH,Leung, FC,Fung, YS,Zhu, DR,Lin, BC,&林炳承.(2005).Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,381(4),812-819.
MLA Qin, JH,et al."Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 381.4(2005):812-819.
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