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题名: Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2
作者: Liang, AY;  He, XY;  Du, YG;  Wang, KY;  Fung, YS;  Lin, BC
通讯作者: 林炳承
关键词: low molecular weight heparin ;  interleukin 2 ;  capillary zone electrophoresis ;  interaction
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2005-07-01
DOI: 10.1016/j.jpba.2005.01.019
卷: 38, 期:3, 页:408-413
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807,1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: A method based on capillary zone electrophoresis (CZE) was used to study the interaction between low molecular weight heparin (LMWH) and interleukin 2 (IL-2). The results showed that the increase of the concentration of LMWH led to the decrease of the peak height and the increase of the peak width of IL-2, but the peak areas were kept constant. The binding constant of IL-2 with LMWH was calculated as 1.2 x 10(6) M(-1) by Scatchard analysis, which is in good agreement with the results found in the references using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the interaction between IL-2 and LMWH is of fast on-and-off kinetic binding reaction. CZE might be used to study not only slow on-and-off rates interactions, but also fast on-and-off rates ones. The binding constant can be calculated easily, and the method can be applied to study a wide range of heparin-protein interactions. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  HUMAN RECOMBINANT INTERLEUKIN-2 ;  AMYLOID-P COMPONENT ;  ASSOCIATION CONSTANTS ;  ANTITHROMBIN-BINDING ;  COMPLEX-FORMATION ;  PROTEIN-BINDING ;  AFFINITY ;  DOMAIN ;  DRUG
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000230235200004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92281
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
4.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Liang, AY,He, XY,Du, YG,et al. Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,38(3):408-413.
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