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题名: Formulation of a basal medium for primary cell culture of the marine sponge Hymeniacidon perleve
作者: Zhao, QY;  Zhang, W;  Jin, MF;  Yu, XJ;  Deng, MC
通讯作者: 张卫
刊名: BIOTECHNOLOGY PROGRESS
发表日期: 2005-05-01
DOI: 10.1021/bp050029c
卷: 21, 期:3, 页:1008-1012
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
英文摘要: Marine sponge cell culture is a potential route for the sustainable production of sponge-derived bioproducts. Development of a basal culture medium is a prerequisite for the attachment, spreading, and growth of sponge cells in vitro. With the limited knowledge available on nutrient requirements for sponge cells, a series of statistical experimental designs has been employed to screen and optimize the critical nutrient components including inorganic salts (ferric ion, zinc ion, silicate, and NaCl), amino acids (glycine, glutamine, and aspartic acid), sugars (glucose, sorbitol, and sodium pyruvate), vitamin C, and mammalian cell medium (DMEM and RPMI 1640) using MTT assay in 96-well plates. The marine sponge Hymeniacidon perleve was used as a model system. Plackett-Burman design was used for the initial screening, which identified the significant factors of ferric ion, NaCl, and vitamin C. These three factors were selected for further optimization by Uniform Design and Response Surface Methodology (RSM), respectively. A basal medium was finally established, which supported an over 100% increase in viability of sponge cells.
关键词[WOS]: FREE AMINO-ACIDS ;  SUBERITES-DOMUNCULA ;  UNIFORM DESIGN ;  IN-VITRO ;  PRIMMORPHS ;  PROLIFERATION ;  EXPRESSION ;  GROWTH ;  PLATES ;  DRUGS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000229605400052
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92333
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Flinders Univ S Australia, CRC Bioprod, Adelaide, SA 5042, Australia
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhao, QY,Zhang, W,Jin, MF,et al. Formulation of a basal medium for primary cell culture of the marine sponge Hymeniacidon perleve[J]. BIOTECHNOLOGY PROGRESS,2005,21(3):1008-1012.
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