DICP OpenIR
Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing
Zhang, XL; Wang, W; Yu, WT; Xie, YB; Zhang, XH; Zhang, Y; Ma, XJ; Ma XJ(马小军); Ma XJ(马小军)
Source PublicationBIOTECHNOLOGY PROGRESS
2005-07-01
ISSN8756-7938
DOI10.1021/bp050003l
Volume21Issue:4Pages:1289-1296
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS KeywordSOLID TUMORS ; CELL-LINES ; CHEMOTHERAPY ; TISSUE ; ASSAY ; RESISTANCE ; CULTURE ; INVITRO ; GROWTH ; MICROCAPSULES
AbstractIn this study, an in vitro multicellular tumor spheroid model was developed using microencapsulation, and the feasibility of using the microencapsulated. multicellular tumor spheroid (MMTS) to test the effect of chemotherapeutic drugs was investigated. Human MCF-7 breast cancer cells were encapsulated in alginate-poly-L-lysine-alginate (APA) microcapsules, and a single multicellular spheroid 150 mu m in diameter was formed in the microcapsule after 5 days of cultivation. The cell morphology, proliferation, and viability of the MMTS were characterized using phase contrast microscopy, BrdU-Iabeling, MTT stain, calcein AM/ED-2 stain, and H&E stain. It demonstrated that the MMTS was viable and that the proliferating cells were mainly localized to the periphery of the cell spheroid and the apoptotic cells were in the core. The MCF-7 MMTS was treated with mitomycin C (MC) at a concentration of 0.1, 1, or 10 times that of peak plasma concentration (ppc) for up to 72 h. The cytotoxicity was demonstrated. clearly by the reduction in cell spheroid size and the decrease in cell viability. The MMTS was further used to screen the anticancer effect of chemotherapeutic drugs, treated with MC, adriamycin (ADM) and 5-fluorouracil (5-FU) at concentrations of 0.1, 1, and 10 ppc for 24, 48, and 72 h. MCF-7 monolayer culture was used as control. Similar to monolayer culture, the cell viability of MMTS was reduced after treatment with anticancer drugs. However, the inhibition rate of cell viability in MMTS was much lower than that in monolayer culture. The MMTS was more resistant to anticancer drugs than monolayer culture. The inhibition rates of cell viability were 68.1%, 45.1%, and 46.8% in MMTS and 95.1%, 86.8%, and 91.6% in monolayer culture treated with MC, ADM, and 5-FU at 10 ppc for 72 h, respectively. MC showed the strongest cytotoxicity in both MMTS and monolayer, followed by 5-FU and ADM. It demonstrated that the MMTS has the potential to be a rapid and valid in vitro model to screen chemotherapeutic drugs with a feature to mimic in vivo three-dimensional (3-D) cell growth pattern.
Language英语
URL查看原文
WOS IDWOS:000231126800036
Citation statistics
Cited Times:118[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92431
Collection中国科学院大连化学物理研究所
Corresponding AuthorMa XJ(马小军); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, XL,Wang, W,Yu, WT,et al. Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing[J]. BIOTECHNOLOGY PROGRESS,2005,21(4):1289-1296.
APA Zhang, XL.,Wang, W.,Yu, WT.,Xie, YB.,Zhang, XH.,...&马小军.(2005).Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing.BIOTECHNOLOGY PROGRESS,21(4),1289-1296.
MLA Zhang, XL,et al."Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing".BIOTECHNOLOGY PROGRESS 21.4(2005):1289-1296.
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