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题名: Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography
作者: Yang, Q;  Liang, Z;  Zhang, LH;  Zhang, XD;  Zhao, JY;  Zhang, WB;  Zhang, YK
通讯作者: 张玉奎
关键词: kallikrein ;  purification ;  chromatography ;  mass spectrometry
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2005-09-15
DOI: 10.1016/j.jpba.2005.05.016
卷: 39, 期:3-4, 页:848-852
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Tissue kallikrein, generally existing in living bodies as prokallikrein, is a serine proteinase that has proven of great significance to treat hypertension, cardiopathy and nephropathy. Although the extraction of tissue kallikrein from human urine is the most commonly used method to obtain such a protein, not only the yield is very little, but also the procedure is rather complex. Furthermore, the biological safety is uncertain. Therefore, the preparation of such a protein by genetic engineering method, including gene expression, cell culture, separation and purification, is very important. In this paper, a new method to obtain purified tissue prokallikrein excreted from insect cells by liquid chromatography has been proposed. In contrast to the previously published papers, the purification procedure is simplified to only three steps with the final yield of 57% and the purity of 95%, which is not only convenient, but also low-cost and suitable for the large-scale preparation of such a protein. The purified protein is further validated as prokallikrein by high performance liquid chromatography-mass spectrometry and amino acid sequencing. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: URINARY KALLIKREIN ;  EXPRESSION ;  RATS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000232209700074
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92489
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Dept Nephrol, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Q,Liang, Z,Zhang, LH,et al. Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,39(3-4):848-852.
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