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题名: Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis
作者: Fu, SP;  Zhang, XZ;  Zhang, F;  Xu, Q;  Xiao, HB;  Liang, XM
通讯作者: 梁鑫淼
关键词: strychnine ;  binding constant ;  capillary electrophoretic frontal analysis ;  bovine serum albumin
刊名: CHINESE CHEMICAL LETTERS
发表日期: 2005-09-01
卷: 16, 期:9, 页:1233-1236
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1804,1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin (BSA) was determined by capillary electrophoretic frontal analysis (CE-FA) for the first time. The experiment was carried out in a polyacrylamide-coated fused silica capillary (48.4 cmx50 mu m i.d., 38.1 cm effective length) with 20 mmol/L citrate/MES buffer (pH 6.0, ionic strength 0.17). The applied voltage was 12 kV and detection wavelength was set at 257 nm. The plateau height of the peak was employed to determine the unbound concentration of drug in BSA equilibrated sample solution based on the external drug standard in the absence of protein. The present method provides a convenient, accurate technique for the early stage of drug screening.
关键词[WOS]: PROTEIN-BINDING
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000231973000027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92491
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Fu, SP,Zhang, XZ,Zhang, F,et al. Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis[J]. CHINESE CHEMICAL LETTERS,2005,16(9):1233-1236.
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