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Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis
Fu, SP; Zhang, XZ; Zhang, F; Xu, Q; Xiao, HB; Liang, XM; Liang XM(梁鑫淼); Liang XM(梁鑫淼)
KeywordStrychnine Binding Constant Capillary Electrophoretic Frontal Analysis Bovine Serum Albumin
Source PublicationCHINESE CHEMICAL LETTERS
2005-09-01
Volume16Issue:9Pages:1233-1236
Indexed BySCI
SubtypeArticle
Department18
Funding Project1804,1803
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordPROTEIN-BINDING
AbstractThe protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin (BSA) was determined by capillary electrophoretic frontal analysis (CE-FA) for the first time. The experiment was carried out in a polyacrylamide-coated fused silica capillary (48.4 cmx50 mu m i.d., 38.1 cm effective length) with 20 mmol/L citrate/MES buffer (pH 6.0, ionic strength 0.17). The applied voltage was 12 kV and detection wavelength was set at 257 nm. The plateau height of the peak was employed to determine the unbound concentration of drug in BSA equilibrated sample solution based on the external drug standard in the absence of protein. The present method provides a convenient, accurate technique for the early stage of drug screening.
Language英语
URL查看原文
WOS IDWOS:000231973000027
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92491
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼); Liang XM(梁鑫淼)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Fu, SP,Zhang, XZ,Zhang, F,et al. Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis[J]. CHINESE CHEMICAL LETTERS,2005,16(9):1233-1236.
APA Fu, SP.,Zhang, XZ.,Zhang, F.,Xu, Q.,Xiao, HB.,...&梁鑫淼.(2005).Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis.CHINESE CHEMICAL LETTERS,16(9),1233-1236.
MLA Fu, SP,et al."Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis".CHINESE CHEMICAL LETTERS 16.9(2005):1233-1236.
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