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Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography
Zhang, Y; Chen, JP; Zhang, CZ; Wu, WZ; Liang, XM; Liang XM(梁鑫淼); Liang XM(梁鑫淼)
KeywordPhytoestrogens Kudzu Root Recombinant Yeast Hplc
Source PublicationJOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
2005-03-01
DOI10.1016/j.jsbmb.2004.10.022
Volume94Issue:4Pages:375-381
Indexed BySCI
SubtypeArticle
Department1,18
Funding Project103,1803
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS KeywordHORMONE REPLACEMENT THERAPY ; LARGE-SCALE USE ; ISOFLAVONES ; FLAVONOIDS ; PUERARIAE ; GENISTEIN ; DAIDZEIN ; EXTRACTS ; RADIX ; RISK
AbstractThe estrogenic activity of the Chinese herb kudzu root was investigated by a recombinant yeast screening assay (YES). Isoflavones are the main components in the plant, of which puerarin is the most abundant one. The kudzu root extract was separated into four fractions according to the polarity. The crude extract and its sub-fractions, except the water fraction, showed clear estrogenic activity and the potencies were in the range of 10(-3) to 10(-1) g/l. The ligand potency was used to compare the estrogenic activity of these fractions. The crude extract and its sub-fractions were further analyzed by high performance liquid chromatography (HPLC) to correlate the activity and the active components. Bioassay and chemical analysis showed that theoretical estrogenic activity expressed as equivalent 17 beta-estradiol concentration or the cumulative effects are comparable to that experimentally determined by YES. The results showed that the high content of isoflavones as well as the high estrogenic activity could make kudzu root extract an interesting candidate for hormone replacement therapy. (c) 2005 Elsevier Ltd. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000229445800011
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92509
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼); Liang XM(梁鑫淼)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Y,Chen, JP,Zhang, CZ,et al. Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,2005,94(4):375-381.
APA Zhang, Y.,Chen, JP.,Zhang, CZ.,Wu, WZ.,Liang, XM.,...&梁鑫淼.(2005).Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography.JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,94(4),375-381.
MLA Zhang, Y,et al."Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography".JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 94.4(2005):375-381.
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