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题名: Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography
作者: Zhang, Y;  Chen, JP;  Zhang, CZ;  Wu, WZ;  Liang, XM
通讯作者: 梁鑫淼
关键词: phytoestrogens ;  kudzu root ;  recombinant yeast ;  HPLC
刊名: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
发表日期: 2005-03-01
DOI: 10.1016/j.jsbmb.2004.10.022
卷: 94, 期:4, 页:375-381
收录类别: SCI
文章类型: Article
部门归属: 1,18
项目归属: 103,1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism
研究领域[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism
英文摘要: The estrogenic activity of the Chinese herb kudzu root was investigated by a recombinant yeast screening assay (YES). Isoflavones are the main components in the plant, of which puerarin is the most abundant one. The kudzu root extract was separated into four fractions according to the polarity. The crude extract and its sub-fractions, except the water fraction, showed clear estrogenic activity and the potencies were in the range of 10(-3) to 10(-1) g/l. The ligand potency was used to compare the estrogenic activity of these fractions. The crude extract and its sub-fractions were further analyzed by high performance liquid chromatography (HPLC) to correlate the activity and the active components. Bioassay and chemical analysis showed that theoretical estrogenic activity expressed as equivalent 17 beta-estradiol concentration or the cumulative effects are comparable to that experimentally determined by YES. The results showed that the high content of isoflavones as well as the high estrogenic activity could make kudzu root extract an interesting candidate for hormone replacement therapy. (c) 2005 Elsevier Ltd. All rights reserved.
关键词[WOS]: HORMONE REPLACEMENT THERAPY ;  LARGE-SCALE USE ;  ISOFLAVONES ;  FLAVONOIDS ;  PUERARIAE ;  GENISTEIN ;  DAIDZEIN ;  EXTRACTS ;  RADIX ;  RISK
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000229445800011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92509
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Y,Chen, JP,Zhang, CZ,et al. Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,2005,94(4):375-381.
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