DICP OpenIR
Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC
Su, XY; Kong, L; Li, X; Chen, XG; Guo, M; Zou, HF; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordBioactive Compounds Biofingerprinting Analysis Small Molecule-dna Binding Microdialysis/hplc Traditional Chinese Medicines
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2005-05-27
ISSN0021-9673
DOI10.1016/j.chroma.2005.04.031
Volume1076Issue:1-2Pages:118-126
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; HUMAN SERUM-ALBUMIN ; BIOLOGICALLY-ACTIVE COMPOUNDS ; PROTEIN INTERACTIONS ; BINDING-PROPERTIES ; ANGELICA-SINENSIS ; TOPOISOMERASE-I ; SAIBOKU-TO ; LIGANDS ; SPECTROSCOPY
AbstractBiofingerprinting chromatogram, analysis, which is defined as the comparison of fingerprinting chromatograms of the extract of traditional Chinese medicines (TCMs) before and after the interaction with biological systems (DNA, protein. cell. etc.), was proposed for screening and analysis of the multiple bioactive compounds in TCMs. A method of microdialysis sampling combined with high performance liquid chromatography (HPLC) was applied to the study of DNA-binding property for the extracts of TCMs. Seven compounds were found to bind to calf thymus DNA (ct-DNA) from the TCMs of Coptis chinensis Franch (Coptis), but only three ones from Phellodendron amurense Rupr. (Phellodendron) and none from Sophoraflavescens Ait. (Sophora) to bind to ct-DNA. respectively. Three of them were identified as berberine, palmatine and jatrorrhizine and their association constants (K) to ct-DNA were determined by microdialysis/HPLC. Competitive binding behaviors of them to ct-DNA were also investigated. © 2005 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000229551000015
Citation statistics
Cited Times:58[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/92763
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China
Recommended Citation
GB/T 7714
Su, XY,Kong, L,Li, X,et al. Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1076(1-2):118-126.
APA Su, XY.,Kong, L.,Li, X.,Chen, XG.,Guo, M.,...&邹汉法.(2005).Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC.JOURNAL OF CHROMATOGRAPHY A,1076(1-2),118-126.
MLA Su, XY,et al."Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC".JOURNAL OF CHROMATOGRAPHY A 1076.1-2(2005):118-126.
Files in This Item:
File Name/Size DocType Version Access License
2005UDX9K84B70.pdf(405KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Su, XY]'s Articles
[Kong, L]'s Articles
[Li, X]'s Articles
Baidu academic
Similar articles in Baidu academic
[Su, XY]'s Articles
[Kong, L]'s Articles
[Li, X]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Su, XY]'s Articles
[Kong, L]'s Articles
[Li, X]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.