DICP OpenIR
Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC
Su, XY; Kong, L; Li, X; Chen, XG; Guo, M; Zou, HF; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Bioactive Compounds Biofingerprinting Analysis Small Molecule-dna Binding Microdialysis/hplc Traditional Chinese Medicines
刊名JOURNAL OF CHROMATOGRAPHY A
2005-05-27
ISSN0021-9673
DOI10.1016/j.chroma.2005.04.031
1076期:1-2页:118-126
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; HUMAN SERUM-ALBUMIN ; BIOLOGICALLY-ACTIVE COMPOUNDS ; PROTEIN INTERACTIONS ; BINDING-PROPERTIES ; ANGELICA-SINENSIS ; TOPOISOMERASE-I ; SAIBOKU-TO ; LIGANDS ; SPECTROSCOPY
英文摘要Biofingerprinting chromatogram, analysis, which is defined as the comparison of fingerprinting chromatograms of the extract of traditional Chinese medicines (TCMs) before and after the interaction with biological systems (DNA, protein. cell. etc.), was proposed for screening and analysis of the multiple bioactive compounds in TCMs. A method of microdialysis sampling combined with high performance liquid chromatography (HPLC) was applied to the study of DNA-binding property for the extracts of TCMs. Seven compounds were found to bind to calf thymus DNA (ct-DNA) from the TCMs of Coptis chinensis Franch (Coptis), but only three ones from Phellodendron amurense Rupr. (Phellodendron) and none from Sophoraflavescens Ait. (Sophora) to bind to ct-DNA. respectively. Three of them were identified as berberine, palmatine and jatrorrhizine and their association constants (K) to ct-DNA were determined by microdialysis/HPLC. Competitive binding behaviors of them to ct-DNA were also investigated. © 2005 Elsevier B.V. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000229551000015
引用统计
被引频次:55[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/92763
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China
推荐引用方式
GB/T 7714
Su, XY,Kong, L,Li, X,et al. Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1076(1-2):118-126.
APA Su, XY.,Kong, L.,Li, X.,Chen, XG.,Guo, M.,...&邹汉法.(2005).Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC.JOURNAL OF CHROMATOGRAPHY A,1076(1-2),118-126.
MLA Su, XY,et al."Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC".JOURNAL OF CHROMATOGRAPHY A 1076.1-2(2005):118-126.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2005UDX9K84B70.pdf(405KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Su, XY]的文章
[Kong, L]的文章
[Li, X]的文章
百度学术
百度学术中相似的文章
[Su, XY]的文章
[Kong, L]的文章
[Li, X]的文章
必应学术
必应学术中相似的文章
[Su, XY]的文章
[Kong, L]的文章
[Li, X]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。