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题名: Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC
作者: Su, XY;  Kong, L;  Li, X;  Chen, XG;  Guo, M;  Zou, HF
通讯作者: 邹汉法
关键词: bioactive compounds ;  biofingerprinting analysis ;  small molecule-DNA binding ;  microdialysis/HPLC ;  traditional Chinese medicines
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2005-05-27
DOI: 10.1016/j.chroma.2005.04.031
卷: 1076, 期:1-2, 页:118-126
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Biofingerprinting chromatogram, analysis, which is defined as the comparison of fingerprinting chromatograms of the extract of traditional Chinese medicines (TCMs) before and after the interaction with biological systems (DNA, protein. cell. etc.), was proposed for screening and analysis of the multiple bioactive compounds in TCMs. A method of microdialysis sampling combined with high performance liquid chromatography (HPLC) was applied to the study of DNA-binding property for the extracts of TCMs. Seven compounds were found to bind to calf thymus DNA (ct-DNA) from the TCMs of Coptis chinensis Franch (Coptis), but only three ones from Phellodendron amurense Rupr. (Phellodendron) and none from Sophoraflavescens Ait. (Sophora) to bind to ct-DNA. respectively. Three of them were identified as berberine, palmatine and jatrorrhizine and their association constants (K) to ct-DNA were determined by microdialysis/HPLC. Competitive binding behaviors of them to ct-DNA were also investigated. © 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  HUMAN SERUM-ALBUMIN ;  BIOLOGICALLY-ACTIVE COMPOUNDS ;  PROTEIN INTERACTIONS ;  BINDING-PROPERTIES ;  ANGELICA-SINENSIS ;  TOPOISOMERASE-I ;  SAIBOKU-TO ;  LIGANDS ;  SPECTROSCOPY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000229551000015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92763
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China

Recommended Citation:
Su, XY,Kong, L,Li, X,et al. Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1076(1-2):118-126.
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