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题名: Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights
作者: Hu, LH;  Chen, XG;  Kong, L;  Su, XY;  Ye, ML;  Zou, HF
通讯作者: 邹汉法
关键词: silica monolithic column ;  low-abundant components ;  traditional Chinese medicines ;  data treatment ;  liquid chromatography ;  comprehensive two-dimensional separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2005-10-28
DOI: 10.1016/j.chroma.2005.06.066
卷: 1092, 期:2, 页:191-198
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Performance of comprehensive two-dimensional liquid chromatography system is greatly improved than we reported previously by using a silica monolithic column as for the second dimensional separation. Due to the increase of the elution speed on the second dimensional monolithic column, the first dimensional column efficiency and analysis rate can be greatly improved as comparing with conventionally second dimensional column. The developed system was applied to analysis of methanol extraction of two umbelliferae herbs Ligusticum chuanxiong Hort. and Angelica sinensis (Oliv.) Diels by using CN column as for the first dimensional separation and a silica monolithic ODS column for the second dimensional separation, and the obtained three-dimensional chromatograms were treated by normalization of peak heights with the value of the highest peak or setting a certain value using a software written in-house. It was observed that much more peaks for low-abundant components in TCM extract can clearly be detected here than we reported before, due to the large difference for the amount of components in TCMs' extract. With the above improvements in separation performance and data treatment, totally about 120 components in methanol extraction of Rhizoma chuanxiong and 100 in A. sinensis were separated with UV detection within 130 min. This result meant that both the number of peaks detected increase twice but the analysis time decease twice if comparing with the previously reported result. (c) 2005 Published by Elsevier B.V.
关键词[WOS]: PHASE LIQUID-CHROMATOGRAPHY ;  CAPILLARY-ZONE-ELECTROPHORESIS ;  MASS-SPECTROMETRY ;  AUTOMATED INSTRUMENTATION ;  ANGELICA-SINENSIS ;  COMPLEX-MIXTURES ;  PEPTIDES ;  PROTEINS ;  CHUANXIONG ;  RESOLUTION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000232510100004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92765
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Hu, LH,Chen, XG,Kong, L,et al. Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1092(2):191-198.
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