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题名: Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE
作者: Zhang, LH;  Zhang, W;  Jin, MF;  Zhang, HY;  Wang, YJ
通讯作者: YunjiWang
关键词: C. reinhardtii SE ;  hydrogenase ;  gene ;  sequence analysis
刊名: PROCESS BIOCHEMISTRY
发表日期: 2005-09-01
DOI: 10.1016/j.procbio.2005.01.017
卷: 40, 期:9, 页:2968-2972
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering
英文摘要: The cDNA of Chlamydomonas reinhardtii SE encoding hydrogenase (HydA2) was obtained from the total RNA of C reinhardtii SE by RT-PCR. The DNA of hydrogenase was amplified by PCR from the genomic DNA of C reinhardtii SE. The cDNA and DNA of hydrogenase were sequenced, respectively. The structure of hydrogenase gene was analyzed by biology software. The open reading frame predicts that the hydrogenase is composed of 3584 bp encoding 505 amino acids in length with a predicted M.W. of 53.69 kDa. Ten exons (including 1518 bp) and nine introns (including 2066 bp) have been found in the hydrogenase, and there were two potential N-glycosylate sites, eight protein kinase C phosphorylation site, eight casein kinase H phosphorylation site and one sulphorylation in the sequence. The theory pI was 6.15. Total number of negatively charged residues (Asp + Glu) and positively charged residues (Arg + Lys) were 55 and 61, respectively. (c) 2005 Elsevier Ltd. All rights reserved.
关键词[WOS]: GREEN-ALGA ;  ENERGY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000231841800006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92783
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Inst Light Ind, Coll Bio & Food Technol, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, LH,Zhang, W,Jin, MF,et al. Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE[J]. PROCESS BIOCHEMISTRY,2005,40(9):2968-2972.
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