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题名: Rapid identification of pathogenic bacteria by capillary electrophoretic analysis of rRNA genes
作者: Zhao, CX;  Xu, GW;  Gao, P;  Yang, J;  Shi, XZ;  Tian, J
通讯作者: 许国旺
关键词: pathogenic bacteria ;  identification ;  capillary electrophoresis ;  rRNA gene
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2005-04-01
DOI: 10.1002/jssc.200400079
卷: 28, 期:6, 页:513-521
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Molecular diagnosis is playing an increasingly important role in the rapid detection and identification of pathogenic organisms in clinical samples. The genetic variation of ribosomal genes in bacteria offers an alternative to culturing for the detection and identification of these organisms. Here 16S rRNA and 16S-23S rRNA spacer region genes were chosen as the amplified targets for single-strand conformation polymorphism (SSCP) and restriction fragment length polymorphism (RFLP) capillary electrophoresis analysis and bacterial identification. The multiple fluorescence based SSCP method for the 16S rRNA gene and the RFLP method for the 16S-23S rRNA spacer region gene were developed and applied to the identification of pathogenic bacteria in clinical samples, in which home-made short-chained linear polyacrylamide (LPA) was used as a sieving matrix; a higher sieving capability and shorter analysis time were achieved than with a commercial sieving matrix because of the simplified template preparation procedure. A set of 270 pathogenic bacteria representing 34 species in 14 genera were analyzed, and a total of 34 unique SSCP patterns representing 34 different pathogenic bacterial species were determined. Based on the use of machine code to represent peak patterns developed in this paper, the identification of bacterial species becomes much easier.
关键词[WOS]: STRAND CONFORMATION POLYMORPHISM ;  POLYMERASE-CHAIN-REACTION ;  DNA FRAGMENTS ;  PCR AMPLIFICATION ;  SIEVING MEDIUM ;  SPACER REGION ;  SEPARATION ;  POLYACRYLAMIDE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000228760400003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92893
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116011, Peoples R China
2.Dalian Ctr Hosp, Dalian 116027, Peoples R China

Recommended Citation:
Zhao, CX,Xu, GW,Gao, P,et al. Rapid identification of pathogenic bacteria by capillary electrophoretic analysis of rRNA genes[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(6):513-521.
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