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题名: Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography
作者: Zheng, YF;  Xu, GW;  Yang, J;  Zhao, XJ;  Pang, T;  Kong, HW
通讯作者: 许国旺
关键词: coupled-column ;  urinary nucleosides ;  high-performance liquid chromatography
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2005-05-05
DOI: 10.1016/j.jchromb.2005.01.033
卷: 819, 期:1, 页:85-90
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A coupled-column liquid chromatographic method for the direct analysis of 14 urinary nucleosides is described. Efficient on-line clean-up and concentration of 14 nucleosides from urine samples were obtained by using a boronic acid-substituted silica column (40 turn x 4.0 mm I.D.) as the first column (Col-1) and a Hypersil ODS2 column (250 mm x 4.6 mm I.D.) as the second column (Col-2). The mobile phases applied consisted of 0.25 mol/L ammonium acetate (pH 8.5) on Col-1, and of 25 mmol/L potassium dihydrogen phosphate (pH 4.5) on Col-2, respectively. Determination of urinary nucleosides was performed on Col-2 column by using a linear gradient elution comprising 25 mmol/L potassium dihydrogen phosphate (pH 4.5) and methanol-water (60:40, v/v) with UV detection at 260 nm. Urinary nucleosides analysis can be carried out by this procedure in 50 min requiring only pH adjustment and the protein precipitation by centrifugation of urine samples. Calibration plots of 14 standard nucleosides showed excellent linearity (r > 0.995) and the limits of detection were at micromolar levels. Both of intra- and inter-day precisions of the method were better than 6.6% for direct determination of 14 nucleosides. The validated method was applied to quantify 14 nucleosides in 20 normal urines to establish reference ranges. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: CANCER-PATIENTS ;  TUMOR-MARKER ;  CAPILLARY-ELECTROPHORESIS ;  AFFINITY-CHROMATOGRAPHY ;  BIOLOGICAL MARKER ;  PSEUDOURIDINE ;  EXCRETION ;  ACID ;  QUANTITATION ;  1-METHYLADENOSINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000228206000012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/92895
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zheng, YF,Xu, GW,Yang, J,et al. Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,819(1):85-90.
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