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Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli
Tan, HD; Dan, GP; Gong, HY; Cao, LJ; Tan HD(谭海东); Tan HD(谭海东)
关键词Affinity Chromatography Gst-fusion Protein Human Interleukin-11
刊名BIOTECHNOLOGY LETTERS
2005-07-01
ISSN0141-5492 (Paper) 1573-6776 (Online)
DOI10.1007/s10529-005-7179-3
27期:13页:905-910
收录类别SCI
文章类型Article
部门归属18
项目归属1816
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]RHEUMATOID-ARTHRITIS ; INTERLEUKIN-11 ; CELLS
英文摘要Mature human interleukin-11 (HuIL-11) is a cytokine consisting of 178 amino acid residues that results from scission of the N-terminal signal peptide, consisting of 21 amino acid residaues, from the corresponding nascent polypeptide. A DNA fragment encoding a truncated HuIL-11 (trHuIL-11), with an additional 5 amino acid residues removed from the N-terminus, was cloned into vector pGEX-2T between the BamHI site and the EcoRI site. Upon transformation with Escherichia coli BL21, the construct over-produced a glutathione S-transferase (GST)-fused protein in a soluble form after IPTG induction. The fusion protein was initially fractionated with butyl-Sepharose 4 fast flow column and by affinity chromatography using a GSH-Sepharose 4B column. On-site enzymatic release with thrombin gave the target protein at 96% purity as judged by SDS-PAGE and HPLC. Expression of the interleukin as a GST-fused protein thus greatly improved downstream processing. Subsequent biological activity assay suggested that trHuIL-11 had similar activity profile to the naturally produced sample and may be a promising candidate for further development as biopharmaceutical.
语种英语
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WOS记录号WOS:000231164000004
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/93187
专题中国科学院大连化学物理研究所
通讯作者Tan HD(谭海东); Tan HD(谭海东)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Duotai Biopharmaceut Co Ltd, Chongqing 200400, Peoples R China
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GB/T 7714
Tan, HD,Dan, GP,Gong, HY,et al. Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2005,27(13):905-910.
APA Tan, HD,Dan, GP,Gong, HY,Cao, LJ,谭海东,&谭海东.(2005).Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli.BIOTECHNOLOGY LETTERS,27(13),905-910.
MLA Tan, HD,et al."Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli".BIOTECHNOLOGY LETTERS 27.13(2005):905-910.
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