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Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia
Chen, XG; Kong, L; Su, XY; Pan, CS; Ye, ML; Zou, HF; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Psoralea Corylifolia Ion-exchange Chromatography Fractionation Lc-apci/ms Maldi-tof/ms Integrated Approach
刊名JOURNAL OF CHROMATOGRAPHY A
2005-09-30
ISSN0021-9673
DOI10.1016/j.chroma.2005.06.067
1089期:1-2页:87-100
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]COUNTER-CURRENT CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINES ; ACTIVE CONSTITUENTS ; CARBON NANOTUBES ; RESOLUTION ; SEPARATION ; PROTEOME ; MOLECULES ; MS ; LC
英文摘要An approach for the separation and identification of components in a traditional Chinese medicine Psoralea corylifolia was developed. Ion-exchange chromatography (IEC) was applied for the fractionation of P corylifolia extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. Each of the enriched fractions was then further separated on an ODS column with detection of UV absorbance and atmospheric pressure chemical ionization mass spectrometer (APCI/MS), respectively, and also analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) with matrix of oxidized carbon nanotubes. Totally more than 188 components in P. corylifolia extract were detected with this integrated approach, and 12 of them were preliminary identified according to their UV spectra and mass spectra performed by APCI/MS and MALDI-TOF/MS. The obtained analytical results not only demonstrated the powerful resolution of integration IEC fractionation with reversed-phase liquid chromatography (RPLC)-APCI/MS and MALDI-TOF/MS for analysis of compounds in a complex sample, but also exhibited the superiority of APCI/MS and MALDI-TOF/MS for identification of low-mass compounds, such as for study of traditional Chinese medicines (TCMs) and metabolome. (c) 2005 Published by Elsevier B.V.
语种英语
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WOS记录号WOS:000231200700012
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/93373
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Chen, XG,Kong, L,Su, XY,et al. Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1089(1-2):87-100.
APA Chen, XG.,Kong, L.,Su, XY.,Pan, CS.,Ye, ML.,...&邹汉法.(2005).Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia.JOURNAL OF CHROMATOGRAPHY A,1089(1-2),87-100.
MLA Chen, XG,et al."Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia".JOURNAL OF CHROMATOGRAPHY A 1089.1-2(2005):87-100.
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