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Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia
Chen, XG; Kong, L; Su, XY; Pan, CS; Ye, ML; Zou, HF; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordPsoralea Corylifolia Ion-exchange Chromatography Fractionation Lc-apci/ms Maldi-tof/ms Integrated Approach
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2005-09-30
ISSN0021-9673
DOI10.1016/j.chroma.2005.06.067
Volume1089Issue:1-2Pages:87-100
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINES ; ACTIVE CONSTITUENTS ; CARBON NANOTUBES ; RESOLUTION ; SEPARATION ; PROTEOME ; MOLECULES ; MS ; LC
AbstractAn approach for the separation and identification of components in a traditional Chinese medicine Psoralea corylifolia was developed. Ion-exchange chromatography (IEC) was applied for the fractionation of P corylifolia extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. Each of the enriched fractions was then further separated on an ODS column with detection of UV absorbance and atmospheric pressure chemical ionization mass spectrometer (APCI/MS), respectively, and also analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) with matrix of oxidized carbon nanotubes. Totally more than 188 components in P. corylifolia extract were detected with this integrated approach, and 12 of them were preliminary identified according to their UV spectra and mass spectra performed by APCI/MS and MALDI-TOF/MS. The obtained analytical results not only demonstrated the powerful resolution of integration IEC fractionation with reversed-phase liquid chromatography (RPLC)-APCI/MS and MALDI-TOF/MS for analysis of compounds in a complex sample, but also exhibited the superiority of APCI/MS and MALDI-TOF/MS for identification of low-mass compounds, such as for study of traditional Chinese medicines (TCMs) and metabolome. (c) 2005 Published by Elsevier B.V.
Language英语
URL查看原文
WOS IDWOS:000231200700012
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/93373
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Chen, XG,Kong, L,Su, XY,et al. Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1089(1-2):87-100.
APA Chen, XG.,Kong, L.,Su, XY.,Pan, CS.,Ye, ML.,...&邹汉法.(2005).Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia.JOURNAL OF CHROMATOGRAPHY A,1089(1-2),87-100.
MLA Chen, XG,et al."Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia".JOURNAL OF CHROMATOGRAPHY A 1089.1-2(2005):87-100.
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