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题名: Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia
作者: Chen, XG;  Kong, L;  Su, XY;  Pan, CS;  Ye, ML;  Zou, HF
通讯作者: 邹汉法
关键词: Psoralea corylifolia ;  ion-exchange chromatography fractionation ;  LC-APCI/MS ;  MALDI-TOF/MS ;  integrated approach
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2005-09-30
DOI: 10.1016/j.chroma.2005.06.067
卷: 1089, 期:1-2, 页:87-100
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An approach for the separation and identification of components in a traditional Chinese medicine Psoralea corylifolia was developed. Ion-exchange chromatography (IEC) was applied for the fractionation of P corylifolia extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. Each of the enriched fractions was then further separated on an ODS column with detection of UV absorbance and atmospheric pressure chemical ionization mass spectrometer (APCI/MS), respectively, and also analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) with matrix of oxidized carbon nanotubes. Totally more than 188 components in P. corylifolia extract were detected with this integrated approach, and 12 of them were preliminary identified according to their UV spectra and mass spectra performed by APCI/MS and MALDI-TOF/MS. The obtained analytical results not only demonstrated the powerful resolution of integration IEC fractionation with reversed-phase liquid chromatography (RPLC)-APCI/MS and MALDI-TOF/MS for analysis of compounds in a complex sample, but also exhibited the superiority of APCI/MS and MALDI-TOF/MS for identification of low-mass compounds, such as for study of traditional Chinese medicines (TCMs) and metabolome. (c) 2005 Published by Elsevier B.V.
关键词[WOS]: COUNTER-CURRENT CHROMATOGRAPHY ;  TRADITIONAL CHINESE MEDICINES ;  ACTIVE CONSTITUENTS ;  CARBON NANOTUBES ;  RESOLUTION ;  SEPARATION ;  PROTEOME ;  MOLECULES ;  MS ;  LC
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000231200700012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93373
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Chen, XG,Kong, L,Su, XY,et al. Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1089(1-2):87-100.
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