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题名: Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis
作者: Chen, XG;  Hu, LH;  Su, XY;  Kong, L;  Ye, ML;  Zou, HF
通讯作者: 邹汉法
关键词: Honeysuckle ;  ion-exchange chromatography fractionation ;  LC-APCI/MS MALDI-TOF/MS ;  integrated approach
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2006-02-24
DOI: 10.1016/j.jpba.2005.07.043
卷: 40, 期:3, 页:559-570
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: A hyphenated method for the isolation and identification of components in a traditional Chinese medicine of Honeysuckle was developed. Ion-exchange chromatography (IEC) was chosen for the fractionation of Honeysuckle extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. Each of the enriched fractions was then further analyzed by reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer (RPLC-APCI/MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) with matrix of oxidized carbon nanotubes, respectively. It can be noted totally more than 117 components were detected by UV detector, APCI/MS and MALDI-TOF/MS in Honeysuckle extract except the, 145 components identified by MALDI-TOF/MS alone with this integrated approach, and 7 of them were preliminary identified according to their UV spectra and mass spectra performed by APCI/MS and MALDI-TOF/MS, respectively. The obtained analytical results not only indicated the approach of integration IEC fractionation with RPLC-APCI/MS and MALDI-TOF/MS is capable of analyzing complex samples, but also exhibited the potential power of the mass spectrometer in detection of low-mass compounds, such as traditional Chinese medicines (TCMs) and complex biological samples. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: COUNTER-CURRENT CHROMATOGRAPHY ;  TRADITIONAL CHINESE MEDICINES ;  ARTEMISIA-CAPILLARIS ;  ACTIVE CONSTITUENTS ;  LONICERA-JAPONICA ;  CARBON NANOTUBES ;  CHLOROGENIC ACID ;  FLOS-LONICERAE ;  IDENTIFICATION ;  RESOLUTION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000235821100010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93379
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Chen, XG,Hu, LH,Su, XY,et al. Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2006,40(3):559-570.
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