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Characterization of interaction property of multicomponents in Chinese Herb with protein by microdialysis combined with HPLC
Guo, M; Su, XY; Kong, L; Li, X; Zou, HF; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordInteraction Traditional Chinese Medicine Protein Microdialysis Hplc
Source PublicationANALYTICA CHIMICA ACTA
2006-01-18
ISSN0003-2670
DOI10.1016/j.aca.2005.07.064
Volume556Issue:1Pages:183-188
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; HUMAN SERUM-ALBUMIN ; METAL-IONS ; BINDING ; CHUANXIONG ; IDENTIFICATION ; SEPARATION ; DELIVERY ; DRUGS ; MS
AbstractInteraction of traditional Chinese Herb Rhizoma Chuanxiong and protein was studied by microdialysis coupled with high performance liquid chromatography. Compounds in Rhizoma Chuanxiong, such as ferulic acid, senkyunolide A and 3-butylphthalide, were identified by HPLC, HPLC-MS and UV-vis. Microdialysis recoveries and binding degrees of compounds in Rhizoma Chuanxiong with human serum albumin (HSA) and other human plasma protein were determined: recoveries of microdialysis sampling ranged from 36.7 to 98.4% with R.S.D. below 3.1%; while binding to HSA ranged from 0 to 91.5% (0.3 mM HSA) and from 0 to 93.5% (0.6 mM HSA), respectively. Compared with HSA, most of compounds bound to human blood serum more extensively and the results showed that binding of these compounds in Rhizoma Chuanxiong was influenced by pH. Two compounds were found to bind to HSA and human blood serum. their binding degrees were consistent with ferulic acid and 3-butylphthalide, the active compounds in Rhizoma Chuanoxiong. (c) 2005 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000234724900023
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/93381
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Dalian Univ Econ & Technol Dev Dist, Dept Chem & Chem Technol, Dalian 116622, Peoples R China
Recommended Citation
GB/T 7714
Guo, M,Su, XY,Kong, L,et al. Characterization of interaction property of multicomponents in Chinese Herb with protein by microdialysis combined with HPLC[J]. ANALYTICA CHIMICA ACTA,2006,556(1):183-188.
APA Guo, M.,Su, XY.,Kong, L.,Li, X.,Zou, HF.,...&邹汉法.(2006).Characterization of interaction property of multicomponents in Chinese Herb with protein by microdialysis combined with HPLC.ANALYTICA CHIMICA ACTA,556(1),183-188.
MLA Guo, M,et al."Characterization of interaction property of multicomponents in Chinese Herb with protein by microdialysis combined with HPLC".ANALYTICA CHIMICA ACTA 556.1(2006):183-188.
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