DICP OpenIR
High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root
Zhang, Y; Xu, Q; Zhang, XZ; Chen, JP; Liang, XM; Kettrup, A; Liang XM(梁鑫淼); Liang XM(梁鑫淼)
KeywordKudzu Root Isoflavones Lc-ms Crude Beta-glycosidase Biotransformation
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2005-11-01
DOI10.1007/s00216-005-0068-8
Volume383Issue:5Pages:787-796
Indexed BySCI
SubtypeArticle
Department18,1
Funding Project1803,103
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPUERARIA-LOBATA ; PHENOLIC GLUCOSIDES ; RED-CLOVER ; PHYTOESTROGENS ; SOY ; QUANTITATION ; ULTRAVIOLET ; SEPARATION ; AGLYCONES ; DAIDZEIN
AbstractHigh-performance liquid chromatography-tandem mass spectrometry has been used to identify isoflavone aglycones and glycosides in kudzu root. Fourteen isoflavones were detected. Among these, six were identified by comparison with authentic standards. Tentative identifications of the other isoflavones are based on UV spectra, mass spectra of protonated and deprotonated molecules, and MS-MS data. Several are reported for the first time in kudzu root. The bioactivity and bioavailability of isoflavone aglycones are usually greater than those of their glycosides. To improve the bioavailability of kudzu root isoflavones, crude beta-glycosidases prepared from microbes were used to hydrolyze the isoflavone glycosides. Several MS modes are combined not only to identify the isoflavones in kudzu root, but also to describe the biotransformation of kudzu root isoflavone glycosides. It is also proved that crude beta-glycosidases have high selectivity toward the O-glycosides of isoflavones.
Language英语
URL查看原文
WOS IDWOS:000233874900014
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/93473
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼); Liang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Tech Univ Munich, D-85354 Freising Weihenstephan, Germany
3.GSF Forschungszentrum Umwelt & Gesundheit GMBH, D-85764 Neuherberg, Germany
Recommended Citation
GB/T 7714
Zhang, Y,Xu, Q,Zhang, XZ,et al. High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(5):787-796.
APA Zhang, Y.,Xu, Q.,Zhang, XZ.,Chen, JP.,Liang, XM.,...&梁鑫淼.(2005).High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,383(5),787-796.
MLA Zhang, Y,et al."High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 383.5(2005):787-796.
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