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题名: Octadecylated silica monolith capillary column with integrated nanoelectrospray ionization emitter for highly efficient proteome analysis
作者: Xie, CH;  Ye, ML;  Jiang, XG;  Jin, WH;  Zou, HF
通讯作者: 邹汉法
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2006-03-01
DOI: 10.1074/mcp.M500272-MCP200
卷: 5, 期:3, 页:454-461
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: An improved strategy for the preparation of octadecylated silica monolith capillary column with high homogeneity was proposed. Column performance was evaluated by nanoscale HPLC. The design for constructing an integrated nanoelectrospray emitter on the octadecylated silica monolith capillary column was first introduced. In comparison with the separated configuration where the emitter is connected to monolithic capillary column by the aid of a zero dead volume union, the integrated capillary column has the inherent advantage of the minimized extracolumn volume thus providing improved separation quality. The performance of the integrated monolithic capillary column was evaluated by separation of BSA tryptic digest, and peak capacity of 313 with a 30-cm column was obtained. The high separation performance allowed highly confident identification of 662 distinct proteins through assignment of 1933 unique peptides by analysis of tryptic digest of 0.5 mu g of Saccharomyces cerevisiae proteins. The higher separation efficiency by a 60-cm monolithic capillary column increased the proteome coverage with identification of 1323 proteins through assignment of 5501 unique peptides over 400-min gradient elution.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  RESONANCE MASS-SPECTROMETRY ;  IDENTIFICATION TECHNOLOGY ;  SEPARATION MEDIA ;  ELECTROCHROMATOGRAPHY ;  ELECTROPHORESIS ;  PROTEINS ;  DATABASE ;  PHASE ;  MS/MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000236142800004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93633
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatogr Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Xie, CH,Ye, ML,Jiang, XG,et al. Octadecylated silica monolith capillary column with integrated nanoelectrospray ionization emitter for highly efficient proteome analysis[J]. MOLECULAR & CELLULAR PROTEOMICS,2006,5(3):454-461.
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