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题名: A normal mucin-binding lectin from the sponge Craniella australiensis
作者: Xiong, CN;  Li, W;  Liu, H;  Zhang, W;  Dou, JL;  Bai, XF;  Du, YG;  Ma, XJ
通讯作者: 李伟
关键词: sponge ;  Craniella australiensis ;  mucin-binding lectin ;  glycoproteins ;  mitogenic
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
发表日期: 2006-05-01
DOI: 10.1016/j.cbpc.2005.11.008
卷: 143, 期:1, 页:9-16
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805,1802,1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism ;  Toxicology ;  Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism ;  Toxicology ;  Zoology
英文摘要: A lectin, Craniella australiensis (CAL), was isolated from sponge C. australiensis by ion-exchange on DEAE-Sephacel and purified by gel filtration on Sephadex G-150 and HPLC on DEAF-5PW. The purified lectin was a trimeric protein as revealed by SDS-PAGE and MALDI-TOF analysis. SDS-PAGE showed that the CAL protein had a molecular mass of 54 kDa, and consisted of three 18 kDa subunits. Gel filtration of purified lectin on Sephadex G-200 indicates that it exists as a 54 kDa protein in its native state. The amino acid composition was rich in Thr and Glx. CAL was found to agglutinate native and trypsinized human A, B erythrocytes, and agglutinate native erythrocytes of mouse, sheep, rabbit and chicken, and trypsinized erythrocytes of sheep and rabbit. The hemagglutination activity was inhibited by glycoproteins such as PSM and asialo-PSM, but not by any of the monosaccharides tested. The activity was stable between 20 and 70 C. Significant CAL activity was observed between pH 5 and 8. The lectin reaction is independent of the presence of divalent cations Ca2+ and Mg2+. The sequence of N-terminal residues of CAL was determined as TSSCQSIVVE. The lectin showed a potent mitogenic response towards BALB/c splenocytes. (c) 2005 Elsevier Inc. All rights reserved.
关键词[WOS]: ASCIDIAN DIDEMNUM-TERNATANUM ;  AXINELLA-POLYPOIDES SCHMIDT ;  AMINO-ACID-SEQUENCE ;  GEODIA-CYDONIUM ;  HUMAN-LYMPHOCYTES ;  SELF RECOGNITION ;  HORSESHOE-CRAB ;  PURIFICATION ;  MOLECULES ;  MITOGEN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237030600002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93659
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Dalian Fisheries Univ, Key & Open Lab Aquat Prod Proc & Utilizat Liaonin, Dalian 116023, Peoples R China

Recommended Citation:
Xiong, CN,Li, W,Liu, H,et al. A normal mucin-binding lectin from the sponge Craniella australiensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2006,143(1):9-16.
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