中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column
作者: Hu, LH;  Li, X;  Feng, S;  Kong, L;  Su, XG;  Chen, XG;  Oln, F;  Ye, ML;  Zou, HF
通讯作者: 邹汉法
关键词: comprehensive two-dimensional separation ;  Rheum palmatum L ;  silica-bonded HSA column ;  silica monolithic ODS column
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2006-04-01
DOI: 10.1002/jssc.20050442
卷: 29, 期:6, 页:881-888
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A mode of comprehensive 2-D LC was developed by coupling a silica-bonded HSA column to a silica monolithic ODS column. This system combined the affinity property of the HSA column and the high-speed separation ability of the monolithic ODS column. The affinity chromatography with HSA-immobilized stationary phase was applied to study the interaction of multiple components in traditional Chinese medicines (TCMs) with HSA according to their affinity to protein in the first dimension. Then the unresolved components retained on the HSA column were further separated on the silica monolithic ODS column in the second dimension. By hyphenating the 2-D separation system to diode array detector and MS detectors, the UV and molecular weight information of the separated compounds can also be obtained. The developed separation system was applied to analysis of the extract of Rheum palmatum L., a number of low-abundant components can be separated on a single peak from the HSA column after normalization of peak heights. Six compounds were preliminarily identified according to their UV and MS spectra. It showed that this system was very useful for biological fingerprinting analysis of the components in TCMs and natural products.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TRADITIONAL CHINESE MEDICINES ;  HUMAN SERUM-ALBUMIN ;  AFFINITY-CHROMATOGRAPHY ;  BIOACTIVE COMPOUNDS ;  RHIZOMA-CHUANXIONG ;  ANGELICA-SINENSIS ;  MASS-SPECTROMETRY ;  SEPARATION ;  PHASE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237162300017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93665
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Hu, LH,Li, X,Feng, S,et al. Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column[J]. JOURNAL OF SEPARATION SCIENCE,2006,29(6):881-888.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Hu, LH]'s Articles
 [Li, X]'s Articles
 [Feng, S]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Hu, LH]‘s Articles
 [Li, X]‘s Articles
 [Feng, S]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace