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Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column
Hu, LH; Li, X; Feng, S; Kong, L; Su, XG; Chen, XG; Oln, F; Ye, ML; Zou, HF; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Comprehensive Two-dimensional Separation Rheum Palmatum l Silica-bonded Hsa Column Silica Monolithic Ods Column
刊名JOURNAL OF SEPARATION SCIENCE
2006-04-01
ISSN1615-9306
DOI10.1002/jssc.20050442
29期:6页:881-888
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINES ; HUMAN SERUM-ALBUMIN ; AFFINITY-CHROMATOGRAPHY ; BIOACTIVE COMPOUNDS ; RHIZOMA-CHUANXIONG ; ANGELICA-SINENSIS ; MASS-SPECTROMETRY ; SEPARATION ; PHASE
英文摘要A mode of comprehensive 2-D LC was developed by coupling a silica-bonded HSA column to a silica monolithic ODS column. This system combined the affinity property of the HSA column and the high-speed separation ability of the monolithic ODS column. The affinity chromatography with HSA-immobilized stationary phase was applied to study the interaction of multiple components in traditional Chinese medicines (TCMs) with HSA according to their affinity to protein in the first dimension. Then the unresolved components retained on the HSA column were further separated on the silica monolithic ODS column in the second dimension. By hyphenating the 2-D separation system to diode array detector and MS detectors, the UV and molecular weight information of the separated compounds can also be obtained. The developed separation system was applied to analysis of the extract of Rheum palmatum L., a number of low-abundant components can be separated on a single peak from the HSA column after normalization of peak heights. Six compounds were preliminarily identified according to their UV and MS spectra. It showed that this system was very useful for biological fingerprinting analysis of the components in TCMs and natural products.
语种英语
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WOS记录号WOS:000237162300017
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/93665
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Hu, LH,Li, X,Feng, S,et al. Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column[J]. JOURNAL OF SEPARATION SCIENCE,2006,29(6):881-888.
APA Hu, LH.,Li, X.,Feng, S.,Kong, L.,Su, XG.,...&邹汉法.(2006).Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column.JOURNAL OF SEPARATION SCIENCE,29(6),881-888.
MLA Hu, LH,et al."Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column".JOURNAL OF SEPARATION SCIENCE 29.6(2006):881-888.
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