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题名: Matrix with high salt tolerance for the analysis of peptide and protein samples by desorption/ionization time-of-flight mass spectrometry
作者: Xu, SY;  Ye, ML;  Xu, DK;  Li, X;  Pan, CS;  Zou, HF
通讯作者: 邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2006-04-15
DOI: 10.1021/ac051572a
卷: 78, 期:8, 页:2593-2599
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: High concentrations of urea and guanidine hydrochloride are commonly used for the denaturation of protein, which was digested by enzymatic proteolysis for the identification by MS analysis. The presence of these contaminants seriously suppresses the ion signal of analytes in MALDI-TOF MS analysis. Herein, a novel MALDI matrix, 3, 4-diaminobenzophenone (DABP), has been found with high tolerance for these contaminants in MALDI MS analysis. The ion signal of analyte insulin can be detected in the presence of 2 M guanidine hydrochloride and 1.5 M urea using DABP as matrix. The tryptic digest of BSA (400 fmol) in 1 M guanidine hydrochloride or 1 M urea was successfully analyzed without any pretreatment prior to MS analysis. Furthermore, it has been found that this matrix can also effectively suppress the cation ion adduction of the peptides in the presence of high concentrations of metal ions in sample solution.
关键词[WOS]: ASSISTED-LASER-DESORPTION/IONIZATION ;  DESORPTION IONIZATION ;  MALDI-MS ;  CARBON NANOTUBES ;  OLIGONUCLEOTIDES ;  ACID ;  MEMBRANE ;  SURFACES ;  SPERMINE ;  SEQUENCE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000236948300017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93715
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Beijing Inst Radiat Med, Dept Biochem, Beijing 100850, Peoples R China

Recommended Citation:
Xu, SY,Ye, ML,Xu, DK,et al. Matrix with high salt tolerance for the analysis of peptide and protein samples by desorption/ionization time-of-flight mass spectrometry[J]. ANALYTICAL CHEMISTRY,2006,78(8):2593-2599.
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